Genome-wide comparative analysis revealed significant transcriptome changes in mice after Toxoplasma gondii infection.
Genome-wide comparative analysis revealed significant transcriptome changes in mice after Toxoplasma gondii infection.
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DOI：
10.1186/1756-3305-6-161
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发表时间：
2013-06-04
影响因子：
3.2
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Jia B;Lu H;Liu Q;Yin J;Jiang N;Chen Q
弓形虫是一种细胞内寄生虫，可以调节宿主的反应和可能的宿主行为。宿主反应以及发病机制取决于负责感染的寄生虫菌株。在免疫功能正常的个体中，T.刚地虫优先感染中枢神经系统（CNS）的组织，这可能是某些精神障碍中的附加因素。而在免疫受损的个体和孕妇中，寄生虫可导致危及生命的感染。随着全基因组研究平台的建立，T。可以系统地调查弓形虫感染。用RH和ME 49株T.将弓形虫以及健康小鼠的弓形虫纯化并用掺入的Cy 5-CTP（实验样品）或Cy 3-CTP（对照样品）转化为cRNA。将标记的cRNA探针与全小鼠基因组微阵列杂交。分析寄生虫感染对脑组织和外周血淋巴细胞基因表达的影响。用实时定量逆转录聚合酶链反应（Q-PCR）对差异表达基因进行再验证。结果表明，两株疟原虫感染后，免疫相关基因表达均上调，但ME 49株感染组小鼠脑组织和外周血淋巴细胞表达均较RH株感染组显著上调。与神经系统发病相关的通路在RH株感染的小鼠中更显著上调。遗传上不同的T.弓形虫株在调节宿主脑组织和外周淋巴细胞的病理生理和免疫反应方面表现出明显的差异。这可能是由于某些宿主对T.弓形虫感染具有普遍性，但免疫反应和中枢神经系统反应具有株系特异性。
Toxoplasma gondii is an intracellular parasite that can modulate host responses and presumably host behavior. Host responses as well as pathogenesis vary depending on the parasite strains that are responsible for infection. In immune competent individuals, T. gondii preferentially infects tissues of the central nervous systems (CNS), which might be an additional factor in certain psychiatric disorders. While in immune-compromised individuals and pregnant women, the parasite can cause life-threatening infections. With the availability of the genome-wide investigation platform, the global responses in gene expression of the host after T. gondii infection can be systematically investigated. Total RNA of brain tissues and peripheral lymphocytes of BALB/C mice infected with RH and ME 49 strain T. gondii as well as that of healthy mice were purified and converted to cRNA with incorporated Cy5-CTP (experimental samples), or Cy3-CTP (control samples). The labeled cRNA probes were hybridized to the Whole Mouse Genome Microarray. The impact of parasite infection on gene expression in both brain tissues and peripheral lymphocytes were analyzed. Differentially expressed genes were revalidated with real-time quantitative reverse transcriptase-polymerase chain reaction (Q-PCR). Data indicated that the genes associated with immunity were up-regulated after infection by the two parasite strains, but significant up-regulation was observed in both brain tissues and peripheral lymphocytes of mice infected with ME49 strain compared to that infected by RH strain. The pathways related to pathogenesis of the nervous system were more significantly up-regulated in mice infected with RH strain. Genetically distinct T. gondii strains showed clear differences in modulation of host pathophysiological and immunological responses in both brain tissue and peripheral lymphocytes. It was likely that some of the host responses to T. gondii infection were universal, but the immune response and CNS reaction were in a strain-specific manner.
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影响因子：
6.4
作者：
Ajzenberg, D;Cogné, N;Dardé, ML
通讯作者：
Dardé, ML
影响因子：
4.4
作者：
Kang, H;Remington, JS;Suzuki, Y
通讯作者：
Suzuki, Y
影响因子：
2.1
作者：
Ferreira, Isabelle M. R.;Vidal, Jose E.;Pereira-Chioccola, Vera L.
通讯作者：
Pereira-Chioccola, Vera L.
影响因子：
6.6
作者：
Carruthers, Vern B.;Suzuki, Yasuhiro
通讯作者：
Suzuki, Yasuhiro
影响因子：
20.3
作者：
Chaussabel, D;Semnani, RT;Nutman, TB
通讯作者：
Nutman, TB