Sodium chloride absorption by the urinary bladder of the winter flounder. A thiazide-sensitive, electrically neutral transport system.
Sodium chloride absorption by the urinary bladder of the winter flounder. A thiazide-sensitive, electrically neutral transport system.
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冬鲽的膀胱吸收氯化钠。
DOI：
10.1172/jci111420
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Stokes,JB
中科院分区：
文献类型：
--
作者：
Stokes,JB
膀胱的冬比目鱼吸收氯化钠的过程中独立的跨上皮电压。与大多数具有中性氯化钠吸收系统的其他上皮细胞相反，比目鱼膀胱具有高的跨上皮阻力。这一特征简化了细胞转运系统的分析，因为离子通过细胞旁途径的转移速率相当低。实验旨在区分中性NaCl吸收的三种可能机制：（a）Na/K/2Cl共运输;（B）平行Na/H和Cl/OH交换;（c）和简单NaCl共运输。Na和Cl对净吸收有明显的相互依赖性。NaCl吸收不依赖于粘膜K，对袢利尿剂敏感性最低。因此，Na/K/2Cl转运系统是不可能的。对阿米洛利和阴离子交换抑制剂4，4 '-二异硫氰基-2，2'-二磺酸二苯乙烯不敏感，证明了该机制不是平行交换。此外，碳酸酐酶抑制剂没有影响。氢氯噻嗪（0.1 mM）几乎完全消除了净吸收。其作用迅速、可逆，且仅从粘膜表面起作用。美托拉宗是苯并噻二嗪类中一种结构不同的利尿剂，具有定性相似的作用。NaCl在该组织中的吸收机制似乎是一个简单的相互依赖的过程。噻嗪类利尿剂的抑制作用似乎是一个独特的特征。比目鱼膀胱可能是远端肾小管吸收NaCl的模型。
The urinary bladder of the winter flounder absorbs NaCl by a process independent of the transepithelial voltage. In contrast to most other epithelia which have a neutral NaCl-absorptive system, the flounder bladder has a high transepithelial resistance. This feature simplifies analysis of the cellular transport system because the rate of ion transfer through the paracellular pathway is rather low. Experiments were designed to distinguish among three possible mechanisms of neutral NaCl absorption: (a) Na/K/2Cl cotransport; (b) parallel Na/H and Cl/OH exchange; (c) and simple NaCl cotransport. A clear interdependency of Na and Cl for net absorption was demonstrated. NaCl absorption was not dependent on mucosal K and was minimally sensitive to loop diuretics. Thus a Na/K/2Cl transport system was unlikely. The mechanism was not parallel exchange as evidenced by insensitivity to amiloride and to 4,4'-diisothiocyano-2,2'-disulfonic stilbene, an inhibitor of anion exchange. In addition, inhibitors of carbonic anhydrase had no effect. Net absorption was almost completely abolished by hydrochlorothiazide (0.1 mM). Its action was rapid, reversible, and effective only from the mucosal surface. Metolazone, a structurally dissimilar diuretic in the benzothiadiazide class had qualitatively similar actions. The mechanism of NaCl absorption in this tissue appears to be a simple interdependent process. Its inhibition by thiazide diuretics appears to be a unique feature. The flounder bladder may be a model for NaCl absorption in the distal renal tubule.
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苯并噻二嗪衍生物对环核苷酸磷酸二酯酶和主动脉带张力的影响。
DOI：
10.1159/000158238
发表时间：
1980
期刊：
Blood vessels
影响因子：
--
作者：
Y. Vulliemoz;M. Verosky;L. Triner
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影响因子：
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作者：
F. Marumo;T. Mishina;H. Shimada
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DOI：
10.1113/jphysiol.1979.sp012926
发表时间：
1979
期刊：
The Journal of Physiology
影响因子：
--
作者：
B. Fossat;B. Lahlou
通讯作者：
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DOI：
10.1152/ajprenal.1982.242.6.f733
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
--
作者：
Warnock,DG;Reenstra,WW;Yee,VJ
通讯作者：
Yee,VJ
DOI：
10.1016/0005-2736(80)90446-0
发表时间：
1980-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
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作者：
GECK, P;PIETRZYK, C;HEINZ, E
通讯作者：
HEINZ, E