Microparticles impact coagulation after traumatic brain injury.
Microparticles impact coagulation after traumatic brain injury.
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DOI：
10.1016/j.jss.2015.02.064
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Goodman MD
中科院分区：
文献类型：
--
作者：
Midura EF;Jernigan PL;Kuethe JW;Friend LA;Veile R;Makley AT;Caldwell CC;Goodman MD
导致创伤性脑损伤(TBI)后常见的亚急性高凝状态的病理生理学机制尚不清楚。颅脑损伤引起的血小板和微粒子(MP)数量和功能的改变被认为是可能的原因；然而，目前对血小板和MPS的作用尚不清楚。采用重型颅脑损伤小鼠模型，采用减重技术。伤后每隔一段时间采血。使用纳米颗粒跟踪分析进行MP计数和表征，并使用血栓弹性仪测定血小板计数和凝血参数。MP促凝血试验用于比较损伤小鼠和假手术小鼠的活性。伤后24小时，循环血小板数无明显变化。然而，血小板对血栓形成的贡献有所减少。相比之下，流通中的MP总数有所下降。当将来自假小鼠的MPS加入到头部受伤动物的血液中时，血小板对凝块形成的贡献趋于正常。相反，当将来自TBI小鼠的MPS添加到假血中时，血小板对血栓形成的贡献显著减少。值得注意的是，在头部受伤的小鼠中，MP促凝血活性增加。颅脑损伤后产生的MPS可能参与了颅脑损伤后凝血功能的改变，并可能在颅脑损伤患者创伤后高凝状态的形成中发挥关键作用。
The pathophysiology that drives the subacute hypercoagulable state commonly seen after traumatic brain injury (TBI) is not well understood. Alterations caused by TBI in platelet and microparticle (MP) numbers and function have been suggested as possible causes; however, the contributions of platelets and MPs are currently unknown. A weight-drop technique of TBI using a murine model of moderate head injury was used. Blood was collected at intervals after injury. MP enumeration and characterization were performed using Nanoparticle Tracking Analysis, and platelet counts and coagulation parameters were determined using thromboelastometry. A MP procoagulant assay was used to compare activity between injured and sham mice. At 24 h after injury, there were no changes in circulating platelet numbers. However, there was a decrease in platelet contribution to clot formation. In contrast, there was a decline in circulating total MP numbers. When MPs from sham mice were added to the blood from head-injured animals, there was a normalization of platelet contribution to clot formation. Conversely, when MPs from TBI mice were added to sham blood, there was a significant decrease in platelet contribution to clot formation. Notably, there was an increase in MP procoagulant activity in head-injured mice. MPs generated after TBI likely contribute to altered coagulation after head injury and may play a key role in the development of a posttraumatic hypercoagulable state in TBI patients.
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影响因子：
3.8
作者：
Park, Myung S.;Owen, Barbara A. L.;Heit, John A.
通讯作者：
Heit, John A.
DOI：
10.1097/ta.0b013e3181991adc
发表时间：
2009-04-01
影响因子：
--
作者：
Haut, Elliott R.;Chang, David C.;Pronovost, Peter J.
通讯作者：
Pronovost, Peter J.
DOI：
10.1097/ta.0b013e31816493ad
发表时间：
2008-03-01
影响因子：
--
作者：
Morel, Nicolas;Morel, Olivier;Dabadie, Philippe
通讯作者：
Dabadie, Philippe
影响因子：
4.2
作者：
Nekludov, Michael;Mobarrez, Fariborz;Wallen, Hakan
通讯作者：
Wallen, Hakan
影响因子：
7.5
作者：
Matijevic, Nena;Wang, Yao-Wei W.;Cohen, Mitchell Jay
通讯作者：
Cohen, Mitchell Jay