Circuit Contributions to Sensory-Driven Glutamatergic Drive of Olfactory Bulb Mitral and Tufted Cells During Odorant Inhalation.
Circuit Contributions to Sensory-Driven Glutamatergic Drive of Olfactory Bulb Mitral and Tufted Cells During Odorant Inhalation.
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DOI：
10.3389/fncir.2021.779056
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发表时间：
2021
影响因子：
3.5
通讯作者：
Wachowiak M
中科院分区：
文献类型：
--
作者：
Moran AK;Eiting TP;Wachowiak M
在哺乳动物嗅球（OB），二尖瓣/簇状（MT）细胞响应气味吸入不同的时间模式，被认为是编码气味信息。这种多样性的大部分已经在MT细胞的突触能输入水平上是明显的，MT细胞从嗅觉感觉神经元（OSN）接收直接的单突触兴奋性输入，以及通过突触能中间神经元的多突触兴奋性驱动。这两种途径也受到OB肾小球层抑制性回路的调节。为了理解直接OSN输入与突触后OB电路机制在形成MT细胞的谷氨酸能驱动的不同动力学中的作用，我们将谷氨酸信号传导成像到麻醉小鼠的MT细胞树突上，同时用离子型谷氨酸受体拮抗剂阻断多突触兴奋性驱动，并用GABAB受体拮抗剂阻断OSN的谷氨酸释放的突触前调节。GABAB受体阻断增加了MT细胞顶端树突上的吸入相关谷氨酸瞬变的幅度，而不改变其吸入相关动力学，证实了突触前抑制影响OSN输入到OB的增益。令人惊讶的是，多突触兴奋的阻断仅适度影响MT细胞的谷氨酸能输入，导致响应于低气味剂浓度的吸入相关谷氨酸瞬变的幅度略有降低，并且每个瞬变的动力学没有变化。突触后阻滞还在较慢的时间尺度上适度影响谷氨酸动力学，主要是通过减少多次吸入气味剂时谷氨酸反应的适应。这些结果表明，从OSNs的直接aminatergic输入提供了大量的兴奋驱动MT细胞，这种输入的动态多样性可能是一个主要的决定因素的MT细胞反应的时间多样性，在这个阶段的气味表示的基础。
In the mammalian olfactory bulb (OB), mitral/tufted (MT) cells respond to odorant inhalation with diverse temporal patterns that are thought to encode odor information. Much of this diversity is already apparent at the level of glutamatergic input to MT cells, which receive direct, monosynaptic excitatory input from olfactory sensory neurons (OSNs) as well as a multisynaptic excitatory drive via glutamatergic interneurons. Both pathways are also subject to modulation by inhibitory circuits in the glomerular layer of the OB. To understand the role of direct OSN input vs. postsynaptic OB circuit mechanisms in shaping diverse dynamics of glutamatergic drive to MT cells, we imaged glutamate signaling onto MT cell dendrites in anesthetized mice while blocking multisynaptic excitatory drive with ionotropic glutamate receptor antagonists and blocking presynaptic modulation of glutamate release from OSNs with GABAB receptor antagonists. GABAB receptor blockade increased the magnitude of inhalation-linked glutamate transients onto MT cell apical dendrites without altering their inhalation-linked dynamics, confirming that presynaptic inhibition impacts the gain of OSN inputs to the OB. Surprisingly, blockade of multisynaptic excitation only modestly impacted glutamatergic input to MT cells, causing a slight reduction in the amplitude of inhalation-linked glutamate transients in response to low odorant concentrations and no change in the dynamics of each transient. The postsynaptic blockade also modestly impacted glutamate dynamics over a slower timescale, mainly by reducing adaptation of the glutamate response across multiple inhalations of odorant. These results suggest that direct glutamatergic input from OSNs provides the bulk of excitatory drive to MT cells, and that diversity in the dynamics of this input may be a primary determinant of the temporal diversity in MT cell responses that underlies odor representations at this stage.
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通过在嗅球肾小球中选择性抑制对二尖瓣/簇状细胞输出的控制。
影响因子：
16.2
作者：
Economo MN;Hansen KR;Wachowiak M
通讯作者：
Wachowiak M
DOI：
10.1523/jneurosci.2368-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gire DH;Schoppa NE
通讯作者：
Schoppa NE
DOI：
10.1523/jneurosci.0154-12.2012
发表时间：
2012-06-06
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Igarashi KM;Ieki N;An M;Yamaguchi Y;Nagayama S;Kobayakawa K;Kobayakawa R;Tanifuji M;Sakano H;Chen WR;Mori K
通讯作者：
Mori K
影响因子：
25
作者：
Haddad, Rafi;Lanjuin, Anne;Madisen, Linda;Zeng, Hongkui;Murthy, Venkatesh N.;Uchida, Naoshige
通讯作者：
Uchida, Naoshige
影响因子：
16.2
作者：
Isaacson, JS
通讯作者：
Isaacson, JS