Elevated solute transport at sites of diffuse matrix damage in cortical bone: Implications on bone repair.
Elevated solute transport at sites of diffuse matrix damage in cortical bone: Implications on bone repair.
复制标题
皮质骨弥漫性基质损伤部位溶质转运升高：对骨修复的影响。
DOI：
10.1002/jor.23742
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Wang B;Sun X;Akkus O;Wang L
已经观察到大鼠皮质骨中的弥漫性基质损伤在两周内有效地自我修复而不激活骨重建，并且与线性裂纹的情况不同，弥漫性损伤部位的局部骨细胞保持健康。然而，基质修复效率如此高的原因仍不清楚。我们假设，运输的矿物质和其他化合物必不可少的损害修复是增强在受损部位，并进一步增加拉伸负荷的应用。为了验证我们的假设，在循环拉伸加载和卸载下，在缺口牛晶片中引入了扩散损伤。使用光漂白后的荧光恢复（FRAP）方法，我们测量了一个小的荧光示踪剂（荧光素钠，376 Da）在受损与未受损的地区和不同的拉伸负荷大小（0.2 N，10 N，20 N，和30 N），这对应于12.5，625，1250，和1875微应变的标称应变，分别运输。我们发现，在受损区域的荧光素运输增加了37%，相对于未受损的区域，在20 N和30 N的张力下，运输进一步增加~18%，与非负载条件相比，可能是由于开裂表面的开口。矿物质和其他粘附蛋白的转运升高可能至少部分地解释了体内观察到的弥漫性损伤的高效修复。临床意义：弥漫性损伤对骨的抗骨折性有不利影响，本研究提供了有关升高的转运的定量数据，其可能参与体内弥漫性损伤的修复。
Diffuse matrix damage in rat cortical bone has been observed to self-repair efficiently in two weeks without activating bone remodeling, and unlike the case with linear cracks, the local osteocytes at the sites of diffuse damage remain healthy. However, the reason(s) for such high efficiency of matrix repair remains unclear. We hypothesized that transport of minerals and other compounds essential for damage repair is enhanced at the damaged sites and further increased by the application of tensile loading. To test our hypothesis, diffuse damage was introduced in notched bovine wafers under cyclic tensile loading and unloading. Using the Fluorescence Recovery After Photobleaching (FRAP) approach, we measured the transport of a small fluorescent tracer (sodium fluorescein, 376 Da) in damaged vs. undamaged regions and under varying tensile load magnitudes (0.2 N, 10 N, 20 N, and 30 N), which corresponded to nominal strains of 12.5, 625, 1250, and 1875 microstrains, respectively. We found a 37% increase in transport of fluorescein in damaged regions relative to undamaged regions and a further ~18% increase in transport under 20N and 30N tension compared to the non-loaded condition, possibly due to the opening of the cracking surfaces. The elevated transport of minerals and other adhesive proteins may, at least partially, account for the highly effective repair of diffuse damage observed in vivo. Clinical Significance: Diffuse damage adversely affects bone’s fracture resistance and this study provided quantitative data on elevated transport, which may be involved in repairing diffuse damage in vivo.
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DOI：
10.1016/j.jmbbm.2013.02.020
发表时间：
2013-05
影响因子：
3.9
作者：
Rivera, C.;Arola, D.;Ossa, A.
通讯作者：
Ossa, A.
影响因子：
6.2
作者：
Price, Christopher;Zhou, Xiaozhou;Li, Wen;Wang, Liyun
通讯作者：
Wang, Liyun
影响因子：
4.1
作者：
Fazzalari, NL;Forwood, MR;Kolesik, P
通讯作者：
Kolesik, P
影响因子：
6.2
作者：
Lu, Yongbo;Yuan, Baozhi;Qin, Chunlin;Cao, Zhengguo;Xie, Yixia;Dallas, Sarah L.;McKee, Marc D.;Drezner, Marc K.;Bonewald, Lynda F.;Feng, Jian Q.
通讯作者：
Feng, Jian Q.
影响因子：
64.8
作者：
PIEKARSKI, K;MUNRO, M
通讯作者：
MUNRO, M