Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion.
Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion.
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DOI：
10.1080/21623945.2023.2282566
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发表时间：
2024-12
期刊：
影响因子：
3.3
通讯作者：
Ma, Yanyan
中科院分区：
文献类型：
--
作者：
Ma, Jiong;Li, Junyan;Chen, Xuejun;Ma, Yanyan
铂是治疗卵巢癌（OvCa）的常用药物，但耐药性限制了其临床应用。本研究旨在描述脂肪细胞对 OvCa 铂电阻的影响。 OvCa 细胞维持在脂肪细胞条件培养基中。分别采用CCK-8和流式细胞术检测细胞活力和凋亡。通过蛋白质印迹法测定增殖和凋亡相关蛋白的表达。使用CCK-8测定顺铂和卡铂的IC50值。分别通过 ELISA 和蛋白质印迹测定 IGF1 分泌和表达。建立异种移植模型，并通过H&E染色检测病理变化。通过 IHC 评估增殖和凋亡相关蛋白表达。脂肪细胞促进OvCa细胞的活力并抑制细胞凋亡，并增强铂类耐药性，而IGF-1 R抑制剂的添加逆转了脂肪细胞对OvCa细胞增殖、凋亡和耐药性的影响。不同浓度的 Ojeok-san (OJS) 处理通过抑制 IGF1 抑制 OvCa 细胞中脂肪细胞诱导的铂耐药。 OJS与顺铂联合治疗可显着抑制体内肿瘤生长，并具有良好的小鼠耐受性。总之，OJS 通过抑制脂肪细胞旁分泌 IGF1 分泌来抑制 OvCa 增殖和铂耐药。
Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum resistance in OvCa. OvCa cells were maintained in the adipocyte-conditioned medium. Cell viability and apoptosis were detected by CCK-8 and flow cytometry, separately. Proliferation and apoptosis-related protein expression were assayed by western blot. The IC50 values of cisplatin and carboplatin were determined using CCK-8. IGF1 secretion and expression were assayed via ELISA and western blot, respectively. A xenograft model was established, and pathological changes were detected by H&E staining. Proliferation and apoptosis-associated protein expression was assessed via IHC. Adipocytes promoted the viability and repressed cell apoptosis in OvCa, as well as enhancing platinum resistance, while the addition of IGF-1 R inhibitor reversed the effects of adipocytes on proliferation, apoptosis, and drug resistance of OvCa cells. Treatment with different concentrations of Ojeok-san (OJS) inhibited the adipocyte-induced platinum resistance in OvCa cells by suppressing IGF1. The combined treatment of OJS and cisplatin significantly inhibited tumour growth in vivo with good mouse tolerance. In summary, OJS inhibited OvCa proliferation and platinum resistance by suppressing adipocyte paracrine IGF1 secretion.
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影响因子：
4.5
作者：
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影响因子：
5.2
作者：
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影响因子：
5.9
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