The effective constituent puerarin, from Pueraria lobata, inhibits the proliferation and inflammation of vascular smooth muscle in atherosclerosis through the miR-29b-3p/IGF1 pathway.
The effective constituent puerarin, from Pueraria lobata, inhibits the proliferation and inflammation of vascular smooth muscle in atherosclerosis through the miR-29b-3p/IGF1 pathway.
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有效的组成puerarin，来自Pueraria lobata，通过miR-29b-3p/igf1途径抑制动脉粥样硬化中血管平滑肌的增殖和炎症。
DOI：
10.1080/13880209.2022.2099430
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发表时间：
2023-12
影响因子：
3.8
通讯作者：
Zhang, Yue
中科院分区：
文献类型：
--
作者：
Li, Jianpeng;Li, Yanan;Yuan, Xiangke;Yao, Dengfeng;Gao, Zongyue;Niu, Zhaoyang;Wang, Zheng;Zhang, Yue
动脉粥样硬化（AS）是心脑血管疾病的主要病因。野葛[专业]植Ohwi（豆科植物）对改善这些疾病有积极作用。本研究旨在探讨野葛对AS血管平滑肌细胞增殖和炎症的影响及其可能的作用机制。通过给8周龄的载脂蛋白E基因敲除小鼠喂食高脂饮食，建立动脉粥样硬化模型。H&E和IHC染色用于分析小鼠的组织病理学。CCK-8、TUNEL和划痕实验分别检测处理24 h后细胞增殖、凋亡和迁移。ELISA法检测IL-6、IL-8水平。通过生物信息学方法筛选出靶miRNA及其下游靶基因，并进行RT-qPCR分析这些基因的表达。葛根素可降低AS小鼠主动脉组织和血清中α-SMA的表达及炎性蛋白IL-6、IL-8的表达。葛根素（200 M）减少hVSMC增殖，迁移，和IL-6和IL-8的分泌超过一半。葛根素对hVSMC的抑制作用被miR-29 b-3 p过表达所减弱。IGF 1是miR-29 b-3 p的下游靶基因。IGF 1在AS小鼠和hVSMC中的表达增加近3倍，但miR-29 b-3 p模拟物抑制IGF 1的表达，当IGF 1过表达时，miR-29 b-3 p对hVSMC的影响被逆转。葛根素通过miR-29 b-3 p/IGF 1通路抑制AS血管平滑肌增殖和炎症反应。葛根素可能在治疗动脉粥样硬化方面具有有益作用，并提供了一种新的治疗选择。
Atherosclerosis (AS) is the main cause of cardiovascular and cerebrovascular diseases. Pueraria lobata (Willd.) Ohwi (Fabaceae) has a positive effect on improving these diseases. The P. lobata effect on the proliferation and inflammation of vascular smooth muscle in AS and the potential mechanism were investigated. By feeding a high-fat diet to 8-week-old apolipoprotein E knockout mice, an atherosclerosis model was created. H&E and IHC staining were used to analyse the histopathology of mice. CCK-8, TUNEL, and scratch tests were used to detect cell proliferation, apoptosis, and migration after 24 h treatment, respectively. ELISA was performed to evaluate the level of IL-6 and IL-8. The target miRNA and its downstream target gene were screened by the bioinformatics method; RT-qPCR has conducted to analyse the expression of these genes. In the aortic tissue and serum of AS mice, puerarin can lower the expression of α-SMA and the inflammatory proteins IL-6 and IL-8. Puerarin (200 M) decreased hVSMC proliferation, migration, and IL-6 and IL-8 secretion by more than half. The inhibitory impact of puerarin on hVSMC was decreased by overexpression of miR-29b-3p. IGF1 was miR-29b-3p's downstream target gene. IGF1 expression increased almost 3-fold in AS mice and hVSMC, but miR-29b-3p mimic inhibited it. The effect of miR-29b-3p on hVSMC was reversed when IGF1 was overexpressed. Puerarin inhibits the proliferation and inflammation of vascular smooth muscle in AS through the miR-29b-3p/IGF1 pathway. Puerarin may have a beneficial effect in the treatment of atherosclerosis and offer a novel therapy option.
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影响因子：
20.1
作者：
Kobiyama K;Ley K
通讯作者：
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作者：
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