GRASP65 controls the cis Golgi integrity in vivo.
GRASP65 controls the cis Golgi integrity in vivo.
复制标题
DOI：
10.1242/bio.20147757
复制
发表时间：
2014-05-02
期刊：
影响因子：
2.4
通讯作者：
Rabouille C
中科院分区：
文献类型：
--
作者：
Veenendaal T;Jarvela T;Grieve AG;van Es JH;Linstedt AD;Rabouille C
GRASP65 and GRASP55 are peripheral Golgi proteins localized to cis and medial/trans cisternae, respectively. They are implicated in diverse aspects of protein transport and structure related to the Golgi complex, including the stacking of the Golgi stack and/or the linking of mammalian Golgi stacks into the Golgi ribbon. Using a mouse model, we interfered with GRASP65 by homologous recombination and confirmed its absence of expression. Surprisingly, the mice were healthy and fertile with no apparent defects in tissue, cellular or subcellular organization. Immortalized MEFs derived from the mice did not show any growth or morphological defects. However, despite the normal appearance of the Golgi ribbon, a fluorescence recovery after photobleaching assay revealed functional discontinuities specific to the cis cisternal membrane network. This leads to a strong change in the plasma membrane GSII lectin staining that was also observed in certain mutant tissues. These findings substantiate the role of GRASP65 in continuity of the cis Golgi network required for proper glycosylation, while showing that neither this continuity nor GRASP65 itself are essential for the viability of a complex organism.
登录
查看更多内容
影响因子：
64.8
作者：
Skarnes WC;Rosen B;West AP;Koutsourakis M;Bushell W;Iyer V;Mujica AO;Thomas M;Harrow J;Cox T;Jackson D;Severin J;Biggs P;Fu J;Nefedov M;de Jong PJ;Stewart AF;Bradley A
通讯作者：
Bradley A
影响因子：
11.4
作者：
Preisinger, C;Körner, R;Barr, FA
通讯作者：
Barr, FA
影响因子：
3.3
作者：
Sütterlin, C;Polishchuk, R;Malhotra, V
通讯作者：
Malhotra, V
影响因子：
7.5
作者：
Giuliani, Fabrizio;Grieve, Adam;Rabouille, Catherine
通讯作者：
Rabouille, Catherine
影响因子：
64.5
作者：
Sütterlin, C;Hsu, P;Malhotra, V
通讯作者：
Malhotra, V