Another triple-spanning envelope protein among intracellularly budding RNA viruses: the torovirus E protein.
Another triple-spanning envelope protein among intracellularly budding RNA viruses: the torovirus E protein.
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DOI：
10.1016/0042-6822(91)90606-c
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发表时间：
1991-06
期刊：
影响因子：
3.7
通讯作者：
Rottier PJ
中科院分区：
文献类型：
--
作者：
Den Boon JA;Snijder EJ;Locker JK;Horzinek MC;Rottier PJ
测定了伯尔尼病毒E蛋白基因的核苷酸序列，并通过体外转录和翻译鉴定了其26.5K翻译产物。对蛋白质序列的计算机分析揭示了III类膜蛋白的特征，其缺乏切割的信号序列，但在N-末端的一半含有三个连续的跨膜α-螺旋，与冠状病毒膜（M）蛋白非常相似。通过在微粒体存在下的体外翻译和随后的蛋白酶K消化研究了E蛋白在膜中的处置。只有小部分的任一端的多肽被发现暴露在囊泡膜的相对侧。用含有冠状病毒M蛋白C-末端尾的杂合E蛋白（EM）进行的实验表明，该C-末端存在于膜的细胞质侧，这是与冠状病毒M蛋白的另一个相似之处。免疫荧光实验表明，EM蛋白，表达的重组痘苗病毒，积累在细胞内膜，主要是那些内质网。圆环病毒E和冠状病毒M蛋白的共同特征支持我们的假设，即这些细胞内萌芽病毒之间存在进化关系。
The nucleotide sequence of the Berne virus envelope (E) protein gene was determined and its 26.5K translation product was identified by in vitro transcription and translation. Computer analysis of the protein sequence revealed the characteristics of a class III membrane protein lacking a cleaved signal sequence but containing three successive transmembrane α-helices in the N-terminal half, much the same as the coronavirus membrane (M) protein. The disposition of the E protein in the membrane was studied by in vitro translation in the presence of microsomes and by subsequent proteinase K digestion. Only small portions of either end of the polypeptide were found to be exposed on opposite sides of the vesicle membranes. Experiments with a hybrid E protein (EM) containing the C-terminal tail of a coronavirus M protein, to which an anti-peptide serum was available, showed that this C-terminus was present at the cytoplasmic side of the membrane, which is another similarity to the coronavirus M protein. Immunofluorescence experiments indicated that the EM protein, expressed by a recombinant vaccinia virus, accumulated in intracellular membranes, predominantly those of the endoplasmic reticulum. The common features of the torovirus E and the coronavirus M protein support our hypothesis that an evolutionary relationship exists between these groups of intracellularly budding viruses.
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影响因子：
3.7
作者：
Kapke PA;Tung FY;Hogue BG;Brian DA;Woods RD;Wesley R
通讯作者：
Wesley R
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
影响因子：
5.4
作者：
MACKETT, M;SMITH, GL;MOSS, B
通讯作者：
MOSS, B
DOI：
10.1073/pnas.85.8.2444
发表时间：
1988-04-01
影响因子：
11.1
作者：
PEARSON, WR;LIPMAN, DJ
通讯作者：
LIPMAN, DJ
影响因子：
3.8
作者：
HORZINEK, MC;EDERVEEN, J;WEISS, M
通讯作者：
WEISS, M