Chemical Exchange Saturation Transfer is Unaffected by Modest Changes in Pressure.
Chemical Exchange Saturation Transfer is Unaffected by Modest Changes in Pressure.
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DOI：
10.1002/ejic.201101291
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发表时间：
2012-04-01
影响因子：
2.3
通讯作者：
Woods, Mark
中科院分区：
文献类型：
--
作者：
Webber, Benjamin C.;Carney, Christiane E.;Woods, Mark
ParaCEST（顺磁性化学交换饱和转移）试剂为通过MRI进行定量分子成像提供了无与伦比的机会。能够响应于局部环境参数（诸如pH、葡萄糖浓度或乳酸盐浓度）的变化而改变它们生成的图像对比度的试剂可以比率地用于定量地描述局部组织环境。然而，当执行这样的定量测量时，重要的是结果不被第二环境参数的变化混淆。体内压力变化相当大，通过呼吸周期和从组织到组织（肿瘤特别具有高的间质压力）。由于paraCEST试剂具有正活化体积，因此它们的交换动力学以及因此它们产生的CEST效应必然与压力相关。本研究的目的是检查在体内表现出的相对较小的压力变化是否会影响CEST足以混淆试图量化其他局部环境参数。在温度范围从288至319 K，在施加的压力范围从0至414 kPa和预饱和（B1）功率范围从524至935 Hz的CEST性能的刚性EuDOTA-四酰胺进行了检查。在任何时候都没有发现压力会影响这种螯合物产生的CEST，这表明体内压力的变化不太可能混淆paraCEST MRI对生理相关参数的定量测量。
ParaCEST (paramagnetic Chemical Exchange Saturation Transfer) agents offer an unparalleled opportunity to perform quantitative molecular imaging by MRI. Agents that can alter the image contrast they generate in response to changes in local environmental parameters such as pH, glucose concentration or lactate concentration can be used ratiometrically to quantitatively describe the local tissue environment. However, when performing such quantitative measurements it is important that the results are not confounded by changes in a second environmental parameter. In vivo pressure varies quite considerably, both through the respiratory cycle and from tissue to tissue (tumors in particular have high interstitial pressures). Since paraCEST agents have positive activation volumes, their exchange kinetics and therefore the CEST effect that they generate are necessarily related to pressure. The purpose of this investigation was to examine whether the relatively small changes in pressure exhibited in vivo could affect CEST sufficiently to confound attempts to quantify other local environmental parameters. The CEST properties of a rigid EuDOTA-tetraamide was examined at temperatures ranging from 288 to 319 K, at applied pressures ranging from 0 to 414 kPa and pre-saturation (B1) powers ranging from 524 to 935 Hz. At no point was pressure found to affect the CEST generated by this chelate, indicating that changes in in vivo pressure is unlikely to confound the quantitative measurement of physiologically relevant parameters by paraCEST MRI.
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影响因子：
4.9
作者：
Aime, S;Barge, A;Puschmann, H
通讯作者：
Puschmann, H
影响因子：
3.3
作者：
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作者：
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影响因子：
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作者：
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