Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray.
Expression profiling of lncRNAs and mRNAs in placental site trophoblastic tumor (PSTT) by microarray.
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DOI：
10.7150/ijms.65002
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发表时间：
2022
影响因子：
3.6
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Gan J;Chen Z;Feng X;Wei Z;Zhang S;Du Y;Xu C;Zhao H
胎盘部位滋养细胞肿瘤（PSTT）起源于中间型滋养细胞，是一种罕见的妊娠滋养细胞疾病。长链非编码RNA（longnoncodingRNA，lncRNA）调控着许多生物学过程。然而，lncRNA在PSTT中的作用仍然知之甚少。在本研究中，lncRNA和mRNA的表达水平在四个人PSTT组织和四个正常胎盘绒毛进行了研究。通过逆转录-实时定量聚合酶反应（RT-qPCR）和免疫组织化学分析验证芯片的结果。此外，进行GO和KEGG通路分析以鉴定异常表达的lncRNA和mRNA的潜在生物学过程和信号传导通路。我们还进行了编码-非编码基因共表达（CNC）网络，以探索改变的lncRNA和mRNA的相互作用。与正常绒毛相比，我们总共鉴定了PSTT组织中1247个上调的lncRNA和1013个下调的lncRNA以及828个上调的mRNA和1393个下调的mRNA（倍数变化≥ 2.0，p <0.05）。GO分析显示，GON是下调最显著的GO项，而免疫应答是上调最显著的GO项。基于六种确认的lncRNA（NONHSAT 114519、NR_103711、NONHSAT 003875、NONHSAT 136587、NONHSAT 134431、NONHSAT 102500）以及354种mRNA的CNC网络图谱由497条边组成。GO和KEGG分析表明，相互作用的mRNA富集在信号识别颗粒（SRP）依赖的共翻译蛋白靶向膜和核糖体途径。本研究有助于加深对PSTT异常lncRNA和mRNAs表达谱的认识，为PSTT的诊断和治疗提供新的思路。
As a rare type of gestational trophoblastic disease, placental site trophoblastic tumor (PSTT) is originated from intermediate trophoblast cells. Long noncoding RNAs (lncRNAs) regulate numerous biological process. However, the role of lncRNAs in PSTT remains poorly understood. In the present study, expression levels of lncRNAs and mRNAs in four human PSTT tissues and four normal placental villi were investigated. The results of microarray were validated by the reverse transcription and quantitative real-time polymerase reaction (RT-qPCR) and immunohistochemistry analyses. Furthermore, GO and KEGG pathway analyses were performed to identify the underlying biological processes and signaling pathways of aberrantly expressed lncRNAs and mRNAs. We also conducted the coding-non-coding gene co-expression (CNC) network to explore the interaction of altered lncRNAs and mRNAs. In total, we identified 1247 up-regulated lncRNAs and 1013 down-regulated lncRNAs as well as 828 up-regulated mRNAs and 1393 down-regulated mRNAs in PSTT tissues compared to normal villi (fold change ≥ 2.0, p < 0.05). GO analysis showed that mitochondrion was the most significantly down-regulated GO term, and immune response was the most significantly up-regulated term. A CNC network profile based on six confirmed lncRNAs (NONHSAT114519, NR_103711, NONHSAT003875, NONHSAT136587, NONHSAT134431, NONHSAT102500) as well as 354 mRNAs was composed of 497 edges. GO and KEGG analyses indicated that interacted mRNAs were enriched in the signal-recognition particle (SRP)-dependent cotranslational protein targeting to membrane and Ribosome pathway. It contributes to expand the understanding of the aberrant lncRNAs and mRNAs profiles of PSTT, which may be helpful for the exploration of new diagnosis and treatment of PSTT.
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通讯作者：
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