The use of alkoxycarbonyl derivatives for the mass spectral analysis of drug-thioether metabolites. Studies with the cysteine, mercapturic acid and glutathione conjugates of acetaminophen.
The use of alkoxycarbonyl derivatives for the mass spectral analysis of drug-thioether metabolites. Studies with the cysteine, mercapturic acid and glutathione conjugates of acetaminophen.
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使用烷氧基羰基衍生物对药物硫醚代谢物进行质谱分析。
DOI：
10.1002/bms.1200151202
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Baillie,TA
中科院分区：
文献类型：
--
作者：
Hoffmann,KJ;Baillie,TA
在缓冲水溶液中制备了对乙酰氨基酚的半胱氨酸-、N-乙酰半胱氨酸-和谷胱甘肽共轭物的烷氧基羰基衍生物，并对其色谱和质谱性质进行了研究。结构信息碎片模式的半胱氨酸和N-乙酰半胱氨酸衍生物时，其甲酯进行分析，通过直接插入化学电离（CH 4）质谱法，虽然需要场解吸和液体二次离子质谱技术，以获得满意的光谱数据的谷胱甘肽加合物的衍生物。用基于N-甲基三氟乙酰胺的甲硅烷基化试剂处理三种对乙酰氨基酚代谢物的乙氧羰基衍生物，导致形成一种共同的挥发性产物，该产物非常适合通过气相色谱/电子轰击质谱法进行分析。提出了一种形成这种新型衍生物的机制，它似乎具有苯并-1，3-噻烷结构，并报道了其质谱特征。最后，讨论了烷氧羰基衍生物用于生物流体中药物-硫醚缀合物分析的实用性，讨论了它们在水相衍生化、高效液相色谱纯化和质谱表征方面的优势。
Alkoxycarbonyl derivatives of the cysteine‐,N‐acetylcysteine‐ and glutathione conjugates of acetaminophen have been prepared in aqueous buffer solutions and their chromatographic and mass spectrometric properties examined. Structurally informative fragmentation patterns of the cysteine‐ andN‐acetylcysteine derivatives were obtained when their methyl esters were subjected to analysis by direct insertion chemical ionization (CH4) mass spectrometry, although field desorption and liquid secondary ion mass spectrometric techniques were required in order to obtain satisfactory spectral data for derivatives of the glutathione adduct. Treatment of ethoxycarbonyl derivatives of the three acetaminophen metabolites withN‐methyltrifluoroacetamide‐based silylating reagents led to the formation of a common volatile product which was ideally suited to analysis by gas chromatography/electron impact mass spectrometry. A mechanism is proposed for the formation of this novel derivative, which appears to possess a benzo‐1,3‐thioxalane structure, and its mass spectral characteristics are reported. Finally, the utility of alkoxycarbonyl derivatives for the analysis of drug—thioether conjugates in biological fluids is discussed in terms of their advantages for aqueous phase derivatization, purification by high‐performance liquid chromatography and characterization by mass spectrometry.
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编码人生长激素释放因子的cDNA的表达克隆和序列
影响因子：
64.8
作者：
K. Mayo;W. Vale;J. Rivier;M. Rosenfeld;R. Evans
通讯作者：
R. Evans
生长调节素/胰岛素样生长因子和胎儿生长。
DOI：
--
发表时间：
1987
期刊：
Journal of developmental physiology
影响因子：
--
作者：
D'Ercole,AJ
通讯作者：
D'Ercole,AJ
人胰岛素样生长因子 I 基因的组织和序列。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rotwein,P;Pollock,KM;Didier,DK;Krivi,GG
通讯作者：
Krivi,GG
影响因子：
4.8
作者：
SHEA, BT;HAMMER, RE;BRINSTER, RL
通讯作者：
BRINSTER, RL
生长调节素的血浆形式和结合蛋白现象。
DOI：
--
发表时间：
1984
期刊：
Clinics in Endocrinology and Metabolism
影响因子：
--
作者：
R. Hintz
通讯作者：
R. Hintz