A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.
A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.
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DOI：
10.1093/jac/dkaa420
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发表时间：
2021-01-19
期刊：
影响因子：
--
通讯作者：
Touw DJ
中科院分区：
文献类型：
--
作者：
Alffenaar JC;Jongedijk EM;van Winkel CAJ;Sariko M;Heysell SK;Mpagama S;Touw DJ
已建议对氟喹诺酮类药物进行个性化给药的治疗药物监测（TDM），以优化疗效并减少MDR TB治疗中的获得性耐药性。因此，本研究的目的是开发一种简单、低成本、稳健的TDM检测方法，使用移动的UV/可见光（UV/维斯）分光光度法在结核病流行环境的护理点定量人唾液中的左氧氟沙星。所有实验均在移动的UV/维斯分光光度计上进行。通过使用7个校准品在300和400 nm之间的二级光谱的振幅来定量左氧氟沙星浓度。使用线性回归根据光谱振幅计算加标样品的浓度。对该方法的选择性、专属性、线性、准确度和精密度进行了验证。检测了频繁联合给药的药物的干扰。左氧氟沙星在2.5-50.0 mg/L范围内线性关系良好，相关系数为0.997。计算的准确度范围为-5.2%至2.4%。总体精密度范围为2.1%-16.1%。应用Savitsky-Golay方法减少了干扰物对左氧氟沙星定量的影响。虽然利福平和吡嗪酰胺在左氧氟沙星浓度的定量下限处显示出分析干扰，但这种干扰对左氧氟沙星剂量的决定没有影响。采用移动的纳米光度计对唾液中左氧氟沙星的紫外/维斯分光光度法进行了验证。该方法可以在程序设置中进行评估，以识别具有低左氧氟沙星药物暴露的患者，从而触发个性化剂量调整。
Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile UV/visible light (UV/VIS) spectrophotometry to quantify levofloxacin in human saliva at the point of care for TB endemic settings. All experiments were performed on a mobile UV/VIS spectrophotometer. The levofloxacin concentration was quantified by using the amplitude of the second-order spectrum between 300 and 400 nm of seven calibrators. The concentration of spiked samples was calculated from the spectrum amplitude using linear regression. The method was validated for selectivity, specificity, linearity, accuracy and precision. Drugs frequently co-administered were tested for interference. The calibration curve was linear over a range of 2.5–50.0 mg/L for levofloxacin, with a correlation coefficient of 0.997. Calculated accuracy ranged from –5.2% to 2.4%. Overall precision ranged from 2.1% to 16.1%. Application of the Savitsky–Golay method reduced the effect of interferents on the quantitation of levofloxacin. Although rifampicin and pyrazinamide showed analytical interference at the lower limit of quantitation of levofloxacin concentrations, this interference had no implication on decisions regarding the levofloxacin dose. A simple UV/VIS spectrophotometric method to quantify levofloxacin in saliva using a mobile nanophotometer has been validated. This method can be evaluated in programmatic settings to identify patients with low levofloxacin drug exposure to trigger personalized dose adjustment.
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影响因子：
4.9
作者：
Peloquin, Charles A.;Phillips, Patrick P. J.;Horsburgh, C. Robert, Jr.
通讯作者：
Horsburgh, C. Robert, Jr.
影响因子：
11.8
作者：
Alffenaar, Jan-Willem C.;Gumbo, Tawanda;Migliori, Giovanni Battista
通讯作者：
Migliori, Giovanni Battista
影响因子：
7.4
作者：
SAVITZKY, A;GOLAY, MJE
通讯作者：
GOLAY, MJE
影响因子：
11.8
作者：
Nahid, Payam;Dorman, Susan E.;Vernon, Andrew
通讯作者：
Vernon, Andrew
DOI：
10.1164/rccm.201909-1874st
发表时间：
2019-11-15
影响因子：
24.7
作者：
Nahid, Payam;Mase, Sundari R.;Seaworth, Barbara
通讯作者：
Seaworth, Barbara