Sulforaphane induced cell cycle arrest in the G2/M phase via the blockade of cyclin B1/CDC2 in human ovarian cancer cells.
Sulforaphane induced cell cycle arrest in the G2/M phase via the blockade of cyclin B1/CDC2 in human ovarian cancer cells.
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硫烷通过人类卵巢癌细胞中的细胞周期蛋白B1/CDC2的阻断在G2/M期诱导细胞周期停滞。
DOI：
10.1186/1757-2215-6-41
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发表时间：
2013-06-26
影响因子：
4
通讯作者：
Hsu YC
中科院分区：
文献类型：
--
作者：
Chang CC;Hung CM;Yang YR;Lee MJ;Hsu YC
恶性肿瘤是最常见的死亡原因，卵巢癌的死亡率在妇科疾病中最高。良性肿瘤切除后通常会完全恢复;然而，即使在肿瘤完全切除后，癌细胞的活性也经常导致快速增殖。因此，临床治疗必须辅以辅助化疗或放疗。萝卜硫素（SFN）是一种来自芥菜家族的提取物，因其抗氧化能力、2相酶诱导和抗肿瘤活性而被公认。本研究采用Western blotting和co-IP Western blotting方法检测SFN对PA-1细胞周期的阻滞作用，以及细胞周期蛋白cyclin B1、Cdc 2和cyclin B1/Cdc 2复合物的表达。本研究以卵巢癌细胞株PA-1为研究对象，探讨膳食异硫氰酸盐SFN对卵巢癌的抗癌作用。SFN处理的细胞通过CDC 2下调和细胞周期蛋白B1/CDC 2复合物的解离在中期积累。我们的研究结果表明，除了已知的预防癌症的作用，SFN也可以提供抗肿瘤活性在建立卵巢癌。
Malignant tumors are the single most common cause of death and the mortality rate of ovarian cancer is the highest among gynecological disorders. The excision of benign tumors is generally followed by complete recovery; however, the activity of cancer cells often results in rapid proliferation even after the tumor has been excised completely. Thus, clinical treatment must be supplemented by auxiliary chemotherapy or radiotherapy. Sulforaphane (SFN) is an extract from the mustard family recognized for its anti-oxidation abilities, phase 2 enzyme induction, and anti-tumor activity. This study investigated the cell cycle arrest in G2/M by SFN and the expression of cyclin B1, Cdc2, and the cyclin B1/CDC2 complex in PA-1 cells using western blotting and co-IP western blotting. This study investigated the anticancer effects of dietary isothiocyanate SFN on ovarian cancer, using cancer cells line PA-1. SFN-treated cells accumulated in metaphase by CDC2 down-regulation and dissociation of the cyclin B1/CDC2 complex. Our findings suggest that, in addition to the known effects on cancer prevention, SFN may also provide antitumor activity in established ovarian cancer.
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影响因子：
3.7
作者：
Drews-Elger K;Ortells MC;Rao A;López-Rodriguez C;Aramburu J
通讯作者：
Aramburu J
DOI：
10.1155/2012/415231
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Chen MJ;Tang WY;Hsu CW;Tsai YT;Wu JF;Lin CW;Cheng YM;Hsu YC
通讯作者：
Hsu YC
影响因子：
4.1
作者：
Biswas, S.;Hwang, J. W.;Rahman, I.
通讯作者：
Rahman, I.
影响因子：
4.2
作者：
Davis, Rachel;Singh, Karan P.;Shankar, Sharmila
通讯作者：
Shankar, Sharmila
影响因子：
--
作者：
Devi, J. Renuka;Thangam, E. Berla
通讯作者：
Thangam, E. Berla