A cytogenetic and genetic characterization of a group of closely linked second chromosome mutations that suppress position-effect variegation in Drosophila melanogaster.
A cytogenetic and genetic characterization of a group of closely linked second chromosome mutations that suppress position-effect variegation in Drosophila melanogaster.
复制标题
一组紧密相连的第二染色体突变的细胞遗传学和遗传特征，这些突变抑制果蝇的位置效应杂色。
作者：
David A. Sinclair;A. Ruddell;J. Brock;N. Clegg;V. Lloyd;T. Grigliatti
一组显性第二染色体位置效应变异（PEV） （Su(var)）突变体的特征揭示了多种有趣的特性，包括：PEV的母系效应抑制、纯合致死性或半致死性和雄性特异性半合子致死性、雌性不育性、对细胞中异染色质数量的急性敏感性和对丁酸钠的敏感性。缺陷/重复定位和互补测试显示，其中8个突变体定义了2号染色体左臂第31节的至少两个基因，并表明第9个突变体对应于该区域内或附近的另一个非必需的Su（var）基因。特异性缺陷和重复对PEV的影响表明，染色体该区域一个或多个Su（var）基因的表达是剂量依赖性的，即能够抑制单倍异常和增强三倍异常。有趣的是，突变体子集中某些可见表型的出现表明它们可能具有反同形性。我们的结果与这两个Su（var）基因编码异染色质结构成分的假设是一致的。我们还报道了先前分离的两个位于31E和31F-32A的突变体作为PEV的隐性抑制因子。
Characterization of a group of dominant second chromosome suppressor of position-effect variegation (PEV) (Su(var)) mutants has revealed a variety of interesting properties, including: maternal-effect suppression of PEV, homozygous lethality or semilethality and male-specific hemizygous lethality, female infecundity, acute sensitivity to the amount of heterochromatin in the cell and sensitivity to sodium butyrate. Deficiency/duplication mapping and complementation tests have revealed that eight of the mutants define at least two genes in section 31 of the left arm of chromosome 2 and they suggest that a ninth corresponds to an additional nonessential Su(var) gene within or near this region. The effects of specific deficiencies and a duplication on PEV indicate that the expression of one or more of the Su(var) genes in this region of the chromosome is dose-dependent, i.e., capable of haplo-abnormal suppression and triplo-abnormal enhancement. Interestingly, the appearance of certain visible phenotypes among a subset of the mutants suggests that they may possess antimorphic properties. Our results are consistent with the hypothesis that two of these Su(var) genes encode structural components of heterochromatin. We also report that two previously isolated mutants located in 31E and 31F-32A act as recessive suppressors of PEV.
childless-abo-like，一种果蝇母体效应突变，在胚盘分裂晚期表现出异常的中心体分离。
DOI：
10.1242/dev.110.2.311
发表时间：
1990
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Sullivan,W;Minden,JS;Alberts,BM
通讯作者：
Alberts,BM
果蝇中位置效应杂色的剂量依赖性修饰剂以及解释其效应的质量作用模型。
影响因子：
3.3
作者：
Locke,J;Kotarski,MA;Tartof,KD
通讯作者：
Tartof,KD
果蝇中 Sgs-4 基因座的多样化表达。
影响因子：
1.6
作者：
Kornher,JS;Kauffman,SA
通讯作者：
Kauffman,SA