Dendrimer-triglycine-EGF nanoparticles for tumor imaging and targeted nucleic acid and drug delivery.
Dendrimer-triglycine-EGF nanoparticles for tumor imaging and targeted nucleic acid and drug delivery.
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DOI：
10.1016/j.oraloncology.2010.07.001
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发表时间：
2010-09
期刊：
影响因子：
4.8
通讯作者：
Yang, Hu
中科院分区：
文献类型：
--
作者：
Yuan, Quan;Lee, Eunmee;Yeudall, W. Andrew;Yang, Hu
关键词：
我们设计了一种含有表皮生长因子 (EGF) 的聚酰胺胺 (PAMAM) 第 4 代树枝状聚合物载体，该载体用量子点标记，用于靶向成像和核酸递送。应用 1H-NMR、SDS-PAGE 和蛋白质印迹来表征合成的 G4.0-GGG-EGF 纳米颗粒。使用 HN12、NIH3T3 和 NIH3T3/EGFR 细胞评估靶向效率、细胞活力、增殖和细胞内信号转导。我们发现，EGF 缀合的树枝状聚合物在选定的浓度下不会刺激 EGFR 表达细胞的生长。与此一致的是，观察到对受体后信号通路的最小刺激。这些纳米颗粒可以以受体依赖性方式定位在表达 EGFR 的细胞内，而缺乏受体的细胞的摄取量较低。使用充分表征的波形蛋白 shRNA (shVIM) 和 siRNA YFP 来测试构建的靶向载体的递送和转染效率。观察到表达的显着降低，表明该载体可用于通过受体靶向机制将核酸或药物引入细胞。
We designed an epidermal growth factor (EGF)-containing polyamidoamine (PAMAM) Generation 4 dendrimer vector labeled with quantum dots for targeted imaging and nucleic acid delivery. 1H-NMR, SDS-PAGE, and western blotting were applied to characterize the synthesized G4.0-GGG-EGF nanoparticles. Targeting efficiency, cell viability, proliferation, and intracellular signal transduction were evaluated using HN12, NIH3T3, and NIH3T3/EGFR cells. We found that EGF-conjugated dendrimers did not stimulate growth of EGFR-expressing cells at the selected concentration. Consistent with this, minimal stimulation of post-receptor signaling pathways was observed. These nanoparticles can localize within cells that express the EGFR in a receptor-dependent manner, whereas uptake into cells lacking the receptor was low. A well characterized vimentin shRNA (shVIM) and siRNA YFP were used to test the delivery and transfection efficiency of the constructed targeted vector. Significant knockdown of expression was observed, indicating that this vector is useful for introduction of nucleic acids or drugs into cells by a receptor-targeted mechanism.
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影响因子：
4.8
作者：
Benke, Emily M.;Ji, Youngmi;Yeudall, W. Andrew
通讯作者：
Yeudall, W. Andrew
DOI：
10.1007/s10856-007-3144-0
发表时间：
2007-10-01
影响因子：
3.7
作者：
Yang, Hu;Lopina, Stephanie T.
通讯作者：
Lopina, Stephanie T.
影响因子：
10.8
作者：
Schatzlein, AG;Zinselmeyer, BH;Uchegbu, IF
通讯作者：
Uchegbu, IF
影响因子：
4.7
作者：
HAENSLER, J;SZOKA, FC
通讯作者：
SZOKA, FC
DOI：
10.1016/s1461-5347(00)00273-x
发表时间：
2000-07-01
期刊：
Pharmaceutical science & technology today
影响因子：
--
作者：
Eichman;Bielinska;Baker Jr
通讯作者：
Baker Jr