Coronavirus infection in chemosensory cells.
Coronavirus infection in chemosensory cells.
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DOI：
10.1007/s13365-023-01117-3
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发表时间：
2023-02
影响因子：
3.2
通讯作者：
Sariyer, Ilker K. K.
中科院分区：
文献类型：
--
作者：
Donadoni, Martina;Kaminski, Rafal;Liao, Shuren;Al Janabi, Suhair;Margolskee, Robert F. F.;Ozdener, Mehmet Hakan;Sariyer, Ilker K. K.
人类冠状病毒（HCoV）相关疾病的临床表现大多与呼吸系统有关，尽管已报告了头痛、嗅觉丧失、味觉丧失和肌痛等继发性并发症。HCoV在与味觉丧失和嗅觉丧失相关的化学感受细胞中的感染和复制知之甚少。在这里，我们的特点是HCoV-OC-43和SARS-CoV-2感染的两种类型的化学感受细胞，嗅觉和味觉细胞，其独特的分子和组织学特征。我们首先在体外培养的人嗅上皮细胞（hOECs）和菌状味觉乳头（HBO）细胞中评估HCoV-OC 43感染。有趣的是，虽然两种细胞类型都对HCoV-OC 43感染敏感，但与hOEC相比，HBO细胞中的病毒复制率显著降低。更有趣的是，虽然来自hOECs的培养基能够在Vero细胞中产生二次感染，但来自HBO细胞的二次感染非常有限，这表明HBO细胞可能无法释放感染性病毒。另一方面，与HCoV-OC 43不同，SARS-CoV-2在hOEC和HBO细胞中显示出相当的病毒感染率水平。此外，我们基于RT-qPCR的基因阵列研究显示，SARS-CoV-2感染显著改变了参与味觉和嗅觉功能的几个关键基因。这些结果可能提示了与SARS-CoV-2感染患者的化学感觉症状（如嗅觉丧失和味觉丧失）相关的可能机制。
Clinical manifestations of human coronavirus (HCoV)-related diseases are mostly related to the respiratory system, although secondary complications such as headache, anosmia, ageusia, and myalgia have been reported. HCoV infection and replication in chemosensory cells associated with ageusia and anosmia is poorly understood. Here, we characterized HCoV-OC43 and SARS-CoV-2 infection in two types of chemosensory cells, olfactory and taste cells, with their unique molecular and histological characteristics. We first assessed HCoV-OC43 infection in in vitro cultured human olfactory epithelial cells (hOECs) and fungiform taste papilla (HBO) cells. Interestingly, while both cell types were susceptible to HCoV-OC43 infection, viral replication rates were significantly reduced in HBO cells compared to hOECs. More interestingly, while culture media from hOECs was able to produce secondary infection in Vero cells, there was very limited secondary infection from HBO cells, suggesting that HBO cells may not be able to release infectious virus. On the other hand, unlike HCoV-OC43, SARS-CoV-2 showed comparable levels of viral infection rates in both hOECs and HBO cells. Furthermore, our RT-qPCR-based gene array studies revealed that several key genes involved in taste and olfactory functions were significantly altered by SARS-CoV-2 infection. These results may suggest a possible mechanism associated with chemosensory symptoms, such as anosmia and ageusia in patients infected with SARS-CoV-2.
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DOI：
10.1016/s1474-4422(20)30308-2
发表时间：
2020-11
期刊：
The Lancet. Neurology
影响因子：
--
作者：
Matschke J;Lütgehetmann M;Hagel C;Sperhake JP;Schröder AS;Edler C;Mushumba H;Fitzek A;Allweiss L;Dandri M;Dottermusch M;Heinemann A;Pfefferle S;Schwabenland M;Sumner Magruder D;Bonn S;Prinz M;Gerloff C;Püschel K;Krasemann S;Aepfelbacher M;Glatzel M
通讯作者：
Glatzel M
影响因子：
3.3
作者：
Borgmann-Winter KE;Rawson NE;Wang HY;Wang H;Macdonald ML;Ozdener MH;Yee KK;Gomez G;Xu J;Bryant B;Adamek G;Mirza N;Pribitkin E;Hahn CG
通讯作者：
Hahn CG
影响因子：
13.6
作者：
通讯作者：
--
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin