The Nup2 meiotic-autonomous region relieves inhibition of Nup60 to promote progression of meiosis and sporulation in Saccharomyces cerevisiae.
The Nup2 meiotic-autonomous region relieves inhibition of Nup60 to promote progression of meiosis and sporulation in Saccharomyces cerevisiae.
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DOI：
10.1093/genetics/iyac045
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发表时间：
2022-05-05
期刊：
影响因子：
3.3
通讯作者：
Burgess, Sean M.
中科院分区：
文献类型：
--
作者：
Komachi, Kelly;Burgess, Sean M.
关键词：
在减数分裂过程中，染色体在结构组织、核定位和运动方面发生了巨大的变化。虽然核孔复合体已被证明影响营养细胞中的基因组组织和功能，但其在减数分裂染色体动力学中的作用仍在很大程度上未被探索。最近在芽殖酵母酿酒酵母中的工作表明，移动的核孔蛋白Nup2是通过减数分裂I前期和孢子形成的正常进展所必需的，在端粒引导的染色体运动已经受损的菌株中。减数分裂自治区是Nup2的一个短片段，负责其在减数分裂中的作用，被证明通过Nup60定位于核膜并与减数分裂染色体结合。为了了解这两种活动对减数分裂自主区功能的相对贡献，我们首先对孢子形成缺陷的减数分裂自主区突变体进行了筛选，发现所有突变都破坏了与Nup60和减数分裂染色体的相互作用。此外，nup60突变体表型复制nup2突变体，表现出类似的核分裂动力学，孢子形成效率，和影响端粒花束突变的遗传相互作用。尽管全长Nup60需要Nup2来发挥功能，但去除Nup60的C末端允许Nup60结合减数分裂染色体并促进没有Nup2的孢子形成。相反，减数分裂自主区与减数分裂染色体的结合完全依赖于Nup60。我们的研究结果揭示了Nup60 C-末端的抑制功能，并表明Nup60介导减数分裂染色体向核膜的募集，而Nup2起着抵消Nup60 C-末端抑制功能的次要作用。
During meiosis, chromosomes undergo dramatic changes in structural organization, nuclear positioning, and motion. Although the nuclear pore complex has been shown to affect genome organization and function in vegetative cells, its role in meiotic chromosome dynamics has remained largely unexplored. Recent work in the budding yeast Saccharomyces cerevisiae demonstrated that the mobile nucleoporin Nup2 is required for normal progression through meiosis I prophase and sporulation in strains where telomere-led chromosome movement has been compromised. The meiotic-autonomous region, a short fragment of Nup2 responsible for its role in meiosis, was shown to localize to the nuclear envelope via Nup60 and to bind to meiotic chromosomes. To understand the relative contribution these 2 activities have on meiotic-autonomous region function, we first carried out a screen for meiotic-autonomous region mutants defective in sporulation and found that all the mutations disrupt interaction with both Nup60 and meiotic chromosomes. Moreover, nup60 mutants phenocopy nup2 mutants, exhibiting similar nuclear division kinetics, sporulation efficiencies, and genetic interactions with mutations that affect the telomere bouquet. Although full-length Nup60 requires Nup2 for function, removal of Nup60’s C-terminus allows Nup60 to bind meiotic chromosomes and promotes sporulation without Nup2. In contrast, binding of the meiotic-autonomous region to meiotic chromosomes is completely dependent on Nup60. Our findings uncover an inhibitory function for the Nup60 C-terminus and suggest that Nup60 mediates recruitment of meiotic chromosomes to the nuclear envelope, while Nup2 plays a secondary role counteracting the inhibitory function in Nup60’s C-terminus.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
7.8
作者：
Dilworth, DJ;Suprapto, A;Aitchison, JD
通讯作者：
Aitchison, JD
影响因子：
3.3
作者：
Chu, Daniel B.;Gromova, Tatiana;Burgess, Sean M.
通讯作者：
Burgess, Sean M.
影响因子：
1.2
作者：
Grubb, Jennifer;Brown, M. Scott;Bishop, Douglas K.
通讯作者：
Bishop, Douglas K.
影响因子：
64.5
作者：
Conrad, Michael N.;Lee, Chih-Ying;Dresser, Michael E.
通讯作者：
Dresser, Michael E.