Proteomic analyses reveal common promiscuous patterns of cell surface proteins on human embryonic stem cells and sperms.
Proteomic analyses reveal common promiscuous patterns of cell surface proteins on human embryonic stem cells and sperms.
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蛋白质组学分析揭示了人胚胎干细胞和精子细胞表面蛋白质的常见混杂模式
DOI：
10.1371/journal.pone.0019386
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发表时间：
2011-05-03
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Gu B;Zhang J;Wu Y;Zhang X;Tan Z;Lin Y;Huang X;Chen L;Yao K;Zhang M
研究背景长期以来，人们一直认为早期胚胎和生殖器官具有相似的基因表达谱。然而，这种相似性是否传播到蛋白质水平仍然是未知的。我们以前的特点是混杂的表达模式的细胞表面蛋白对小鼠胚胎干细胞（mES）。由于细胞表面蛋白在人胚胎干细胞和生殖细胞中也发挥着重要作用，因此揭示这些细胞是否也存在混杂的细胞表面蛋白模式是很重要的。方法和主要发现hES细胞和人成熟精子（hSperms）的表面蛋白用生物素标记纯化，并进行蛋白质组学分析。在两种细胞类型上分别鉴定了超过1000种跨膜或分泌的细胞表面蛋白。这两种细胞类型的蛋白质涵盖了各种各样的功能类别，包括信号转导，粘附和运输。此外，这两种细胞类型混杂地表达了多种组织特异性表面蛋白，并且一些表面蛋白是异质表达的。结论/意义我们的研究结果表明，功能和组织特异性细胞表面蛋白的混杂表达可能是胚胎干细胞和生殖细胞的共同模式。早期胚胎细胞和生殖细胞之间基因表达模式的保守性被传播到蛋白质水平。这些结果对多能干细胞和生殖细胞的细胞表面特征表征具有深刻的意义，并可能导致一个新的研究领域，即，多能细胞和生殖细胞中混杂基因表达的功能意义。
Background It has long been proposed that early embryos and reproductive organs exhibit similar gene expression profiles. However, whether this similarity is propagated to the protein level remains largely unknown. We have previously characterised the promiscuous expression pattern of cell surface proteins on mouse embryonic stem (mES) cells. As cell surface proteins also play critical functions in human embryonic stem (hES) cells and germ cells, it is important to reveal whether a promiscuous pattern of cell surface proteins also exists for these cells. Methods and Principal Findings Surface proteins of hES cells and human mature sperms (hSperms) were purified by biotin labelling and subjected to proteomic analyses. More than 1000 transmembrane or secreted cell surface proteins were identified on the two cell types, respectively. Proteins from both cell types covered a large variety of functional categories including signal transduction, adhesion and transporting. Moreover, both cell types promiscuously expressed a wide variety of tissue specific surface proteins, and some surface proteins were heterogeneously expressed. Conclusions/Significance Our findings indicate that the promiscuous expression of functional and tissue specific cell surface proteins may be a common pattern in embryonic stem cells and germ cells. The conservation of gene expression patterns between early embryonic cells and reproductive cells is propagated to the protein level. These results have deep implications for the cell surface signature characterisation of pluripotent stem cells and germ cells and may lead the way to a new area of study, i.e., the functional significance of promiscuous gene expression in pluripotent and germ cells.
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DOI：
10.1084/jem.20092144
发表时间：
2010-05-10
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Nishikawa Y;Hirota F;Yano M;Kitajima H;Miyazaki J;Kawamoto H;Mouri Y;Matsumoto M
通讯作者：
Matsumoto M
胚胎干细胞中细胞表面蛋白的整体表达
影响因子：
3.7
作者：
Gu B;Zhang J;Wang W;Mo L;Zhou Y;Chen L;Liu Y;Zhang M
通讯作者：
Zhang M
影响因子：
64.5
作者：
Kim, Jonghwan;Chu, Jianlin;Orkin, Stuart H.
通讯作者：
Orkin, Stuart H.
影响因子：
5.2
作者：
Choo, Andre B.;Tan, Heng Liang;Yap, Miranda
通讯作者：
Yap, Miranda
影响因子：
5.2
作者：
Brimble, Sandii N.;Sherrer, Eric S.;Schulz, Thomas C.
通讯作者：
Schulz, Thomas C.