Chitosan Oleate Coated PLGA Nanoparticles as siRNA Drug Delivery System.
Chitosan Oleate Coated PLGA Nanoparticles as siRNA Drug Delivery System.
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DOI：
10.3390/pharmaceutics13101716
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发表时间：
2021-10-17
期刊：
影响因子：
5.4
通讯作者：
Bonferoni MC
中科院分区：
文献类型：
--
作者：
Miele D;Xia X;Catenacci L;Sorrenti M;Rossi S;Sandri G;Ferrari F;Rossi JJ;Bonferoni MC
寡核苷酸治疗剂如miRNA和siRNA代表一类被开发用于通过干扰核糖核酸（RNA）和蛋白质合成来调节基因表达的分子。这些分子的特点是不稳定性强，容易被核酸酶降解。为了避免这些缺点并确保有效递送至靶细胞，病毒和非病毒载体是目前采用的两种主要方法。病毒载体是基因治疗的主要载体之一，然而，强免疫原性和插入突变是患者的潜在问题。非病毒载体，如聚合物纳米载体，提供RNA干扰分子的更安全和更有效的递送。本工作的目的是采用壳聚糖油酸酯包覆的PLGA核纳米颗粒作为siRNA载体。使用靶向HIV-1、针对病毒达特/Rev转录物的siRNA作为模型。利用带负电荷的寡核苷酸部分与壳聚糖壳的正表面电荷之间的离子相互作用来关联siRNA和纳米颗粒。非共价键可以保护siRNA免受核酸酶降解，并保证良好的细胞内化和siRNA快速释放到胞质部分，使其易于活化。
Oligonucleotide therapeutics such as miRNAs and siRNAs represent a class of molecules developed to modulate gene expression by interfering with ribonucleic acids (RNAs) and protein synthesis. These molecules are characterized by strong instability and easy degradation due to nuclease enzymes. To avoid these drawbacks and ensure efficient delivery to target cells, viral and non-viral vectors are the two main approaches currently employed. Viral vectors are one of the major vehicles in gene therapy; however, the potent immunogenicity and the insertional mutagenesis is a potential issue for the patient. Non-viral vectors, such as polymeric nanocarriers, provide a safer and more efficient delivery of RNA-interfering molecules. The aim of this work is to employ PLGA core nanoparticles shell-coated with chitosan oleate as siRNA carriers. An siRNA targeted on HIV-1, directed against the viral Tat/Rev transcripts was employed as a model. The ionic interaction between the oligonucleotide’s moieties, negatively charged, and the positive surface charges of the chitosan shell was exploited to associate siRNA and nanoparticles. Non-covalent bonds can protect siRNA from nuclease degradation and guarantee a good cell internalization and a fast release of the siRNA into the cytosolic portion, allowing its easy activation.
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影响因子：
64.8
作者：
Castanotto, Daniela;Rossi, John J.
通讯作者：
Rossi, John J.
影响因子：
5.4
作者：
Baxi, Krishna;Sawarkar, Sujata;Fernandes, Trinette
通讯作者：
Fernandes, Trinette
AIE 特色无机-有机核@壳纳米粒子，用于高效 siRNA 传输和实时监测
影响因子：
10.8
作者：
He, Xuewen;Yin, Feng;Tang, Ben Zhong
通讯作者：
Tang, Ben Zhong
影响因子：
4.6
作者：
Khan, Azmat Ali;Alanazi, Amer M.;Ansari, Mohammad Azam
通讯作者：
Ansari, Mohammad Azam
DOI：
10.1016/b978-0-08-100230-8.00003-0
发表时间：
2017-01-01
期刊：
CHITOSAN BASED BIOMATERIALS, VOL 1: FUNDAMENTALS
影响因子：
--
作者：
Hoemann, C. D.;Fong, D.
通讯作者：
Fong, D.