Sevoflurane Does Not Promote the Colony-Forming Ability of Human Mesenchymal Glioblastoma Stem Cells In Vitro.
Sevoflurane Does Not Promote the Colony-Forming Ability of Human Mesenchymal Glioblastoma Stem Cells In Vitro.
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DOI：
10.3390/medicina58111614
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发表时间：
2022-11-08
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背景和目标：据报道，临床使用浓度的七氟烷（一种吸入麻醉剂）可显著抑制肿瘤生长。我们研究了七氟烷对人胶质母细胞瘤干细胞（GSC）球体形成和增殖的影响，以确定七氟烷是否对人肿瘤细胞产生短期和长期影响。材料和方法：将高级别患者来源的GSC（MD 13和Me 83）暴露于2%七氟烷。为了评价七氟烷对活力、增殖和干性的影响，我们进行了caspase-3/7试验、细胞增殖试验和有限稀释球形成试验。使用定量逆转录PCR评估上皮肿瘤中癌干细胞样细胞的细胞表面标志物CD 44的表达。采用单因素方差分析（ANOVA）评价组间差异。结果如下：七氟烷暴露4天未显著促进MD 13和Me 83中的caspase 3/7活性，暴露5天后未观察到细胞增殖。此外，长时间暴露于七氟烷6天并未促进MD 13和Me 83细胞的球体形成和增殖潜力。这些结果表明，七氟烷不会促进体外人间充质胶质母细胞瘤干细胞的凋亡、增殖能力或集落形成能力。结论：临床使用浓度的七氟烷不会促进体外人间充质胶质母细胞瘤干细胞的集落形成能力。七氟烷是一种用于外科麻醉的挥发性麻醉剂，它不会加重GSC的病程，这对神经外科医生和麻醉医生来说非常重要。
Background and Objectives: Clinically used concentrations of sevoflurane, an inhaled anesthetic, have been reported to significantly inhibit tumor growth. We investigated the effects of sevoflurane on sphere formation and the proliferation of human glioblastoma stem cells (GSCs) to determine whether sevoflurane exerts short- and long-term effects on human tumor cells. Materials and Methods: High-grade patient-derived GSCs (MD13 and Me83) were exposed to 2% sevoflurane. To evaluate the effect of sevoflurane on viability, proliferation, and stemness, we performed a caspase-3/7 essay, cell proliferation assay, and limiting dilution sphere formation assays. The expression of CD44, a cell surface marker of cancer stem-like cells in epithelial tumors, was evaluated using quantitative reverse transcription PCR. Differences between groups were evaluated with a one-way analysis of variance (ANOVA). Results: Sevoflurane exposure for 4 days did not significantly promote caspase 3/7 activity in MD13 and Me83, and cell proliferation was not observed after 5 days of exposure. Furthermore, prolonged exposure to sevoflurane for 6 days did not promote the sphere-forming and proliferative potential of MD13 and Me83 cells. These results suggest that sevoflurane does not promote either apoptosis, proliferative capacity, or the colony-forming ability of human mesenchymal glioblastoma stem cells in vitro. Conclusions: Sevoflurane at clinically used concentrations does not promote the colony-forming ability of human mesenchymal glioblastoma stem cells in vitro. It is very important for neurosurgeons and anesthesiologists to know that sevoflurane, a volatile anesthetic used in surgical anesthesia, would not exacerbate the disease course of GSCs.
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DOI：
10.3390/medicina57010045
发表时间：
2021-01-07
期刊：
Medicina (Kaunas, Lithuania)
影响因子：
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作者：
Kim Y;Yun S;Shin KA;Chung W;Ko Y;Kim YH;Lim C
通讯作者：
Lim C
影响因子：
6.1
作者：
Han, Xue-Chang;Zhang, Ya-Jie;Zhang, Lu
通讯作者：
Zhang, Lu
影响因子：
4.6
作者：
Sumi C;Okamoto A;Tanaka H;Kusunoki M;Shoji T;Uba T;Adachi T;Iwai T;Nishi K;Harada H;Bono H;Matsuo Y;Hirota K
通讯作者：
Hirota K
影响因子：
5
作者：
Fan, Lihua;Wu, Yini;Han, Xin
通讯作者：
Han, Xin
影响因子：
--
作者：
Iwasaki M;Zhao H;Jaffer T;Unwith S;Benzonana L;Lian Q;Sakamoto A;Ma D
通讯作者：
Ma D