Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro.
Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro.
复制标题
DOI：
10.3390/medicina57010045
复制
发表时间：
2021-01-07
期刊：
影响因子：
--
通讯作者：
Lim C
中科院分区：
文献类型：
--
作者：
Kim Y;Yun S;Shin KA;Chung W;Ko Y;Kim YH;Lim C
背景和目标：有几项研究表明七氟烷可促进癌细胞增殖，而其他研究表明对临床结局无影响。我们进行了体内和体外实验，以研究七氟醚，一种挥发性麻醉剂，对刘易斯肺癌（LLC）细胞的增殖和结果的影响。材料与方法：共37只小鼠注射LLC细胞，比较七氟烷暴露组（sevo组）和对照组的肿瘤大小和生存率。Sevo组暴露于2%七氟烷和4 L/min氧气，每天1 h，每周3次，对照组仅暴露于4 L/min氧气。体外研究中，12个平板与LCC细胞一起孵育。将6个平板暴露于2%七氟烷，持续3天，每天1小时，6个平板未暴露，并在3天后比较细胞增殖。结果如下：暴露于七氟烷的小鼠和对照小鼠之间的存活率或肿瘤大小无显著差异（存活率：29.06 ± 4.45 vs. 28.76 ± 3.75，p = 0.836;肿瘤大小：0.75（0.41-1.02）vs. 0.49（0.11-0.79），p = 0.153）。然而，在体外研究中，与对照组相比，暴露于七氟烷的LLC细胞增殖增加了9.2%（p = 0.018）。结论：七氟烷（2 vol%）暴露可促进LLC细胞在体外环境中的增殖，但可能不影响LLC细胞在体内环境中的增殖。这些结果表明，在体外研究的麻醉剂对癌症的影响可能不同于在体内或临床研究。
Background and objectives: There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and outcomes of Lewis lung carcinoma (LLC) cells. Materials and Methods: A total of 37 mice were injected with LLC cells to compare the tumor size and survival of the sevoflurane exposed group (sevo group) and control group. The sevo group was exposed to 2% sevoflurane and 4 L/min of oxygen for 1 h per day 3 times per week, and the control group was exposed only to 4 L/min of oxygen. In vitro study, 12 plates incubated with LCC cells. 6 plates were exposed to 2% sevoflurane for 1 hr/day for 3 days and 6 plates were not exposed, and cell proliferation was compared after 3 days. Results: There were no significant differences in survival or tumor size between mice exposed to sevoflurane and control mice (survival: 29.06 ± 4.45 vs. 28.76 ± 3.75, p = 0.836; tumor size: 0.75 (0.41–1.02) vs. 0.49 (0.11–0.79), p = 0.153). However, in vitro study, the proliferation of LLC cells exposed to sevoflurane increased by 9.2% compared to the control group (p = 0.018). Conclusions: Sevoflurane (2 vol%) exposure could promote proliferation of LLC cells in vitro environment, but may not affect proliferation of LLC cells in vivo environment. These results suggest that in vitro studies on the effects of anesthetics on cancer may differ from those of in vivo or clinical studies.
登录
查看更多内容
影响因子：
--
作者：
Iwasaki M;Zhao H;Jaffer T;Unwith S;Benzonana L;Lian Q;Sakamoto A;Ma D
通讯作者：
Ma D
影响因子：
3.8
作者：
Lim, Jeong-Ae;Oh, Chung-Sik;Kim, Seong-Hyop
通讯作者：
Kim, Seong-Hyop
影响因子：
9.8
作者：
Luo, X.;Zhao, H.;Ma, D.
通讯作者：
Ma, D.
影响因子：
3
作者：
TOMAYKO, MM;REYNOLDS, CP
通讯作者：
REYNOLDS, CP
影响因子：
3.8
作者：
Qin RS;Zhang ZH;Zhu NP;Chen F;Guo Q;Hu HW;Fu SZ;Liu SS;Chen Y;Fan J;Han YW
通讯作者：
Han YW