Microwaves and the cell membrane. II. Temperature, plasma, and oxygen mediate microwave-induced membrane permeability in the erythrocyte.
Microwaves and the cell membrane. II. Temperature, plasma, and oxygen mediate microwave-induced membrane permeability in the erythrocyte.
复制标题
微波和细胞膜。
DOI：
10.2307/3576466
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发表时间：
1985
影响因子：
3.4
通讯作者：
P. Vanĕk
中科院分区：
文献类型：
--
作者：
R. Liburdy;P. Vanĕk
微波（2450 MHz）显示，以增加22 Na的兔红细胞的渗透性，暴露仅在17.7至19.5摄氏度（Tc）的窄温度范围内，这与反映了明显的膜相变的Arrhenius图中的非线性相一致。值得注意的是，这种反应没有观察到胆固醇负载的红细胞膜，表现出线性Arrhenius图和没有明显的相变在Tc。在Tc处的磁导率增加是吸收功率的非线性函数，但是是样品的内部电场强度的线性函数，并且分别在约400 mW/g和600 V/m处饱和。渗透性的增加被认为是可逆的和短暂的，立即终止后，暴露钠流入显着减少，但在60分钟内恢复正常。细胞外因素对微波效应产生显着影响。血浆的存在显着加强在Tc的22 Na渗透性的增加。氧气也调节微波效应，相对低氧（5 mm Hg）和高氧（760 mm Hg）增强渗透性增加。相反，两种抗氧化剂，抗坏血酸或巯基乙醇的存在下，抑制效果。这些发现提出了重要的问题，微波与细胞膜相互作用的物理和化学性质，也揭示了早期的研究报告，无论是积极的或消极的影响膜渗透性。
Microwaves (2450 MHz) are shown to increase 22Na permeability of rabbit erythrocytes for exposures only within the narrow temperature range of 17.7 to 19.5 degrees C (Tc) which coincides with a nonlinearity in the Arrhenius plot reflecting an apparent membrane phase transition. Significantly, this response is not observed for cholesterol-loaded erythrocyte membranes which exhibit a linear Arrhenius plot and no apparent phase transition at Tc. The permeability increase at Tc is a nonlinear function of absorbed power but is a linear function of the internal electric field strength of the sample and saturates at approximately 400 mW/g and 600 V/m, respectively. The permeability increase was found to be reversible and transient in that immediately following termination of exposure sodium influx is significantly reduced but returns to normal within 60 min. Extracellular factors exert a significant influence on the microwave effect. The presence of plasma markedly potentiates the increase in 22Na permeability at Tc. Oxygen also modulates the microwave effect with relative hypoxia (5 mm Hg) and hyperoxia (760 mm Hg) enhancing the permeability increase. In contrast, the presence of two antioxidants, ascorbic acid or mercaptoethanol, inhibits the effect. These findings raise important questions about the physical and chemical nature of microwave interactions with cell membranes and also shed light on earlier studies reporting either positive or negative effects on membrane permeability.
影响因子：
56.9
作者：
AMES, BN
通讯作者：
AMES, BN