Cis-acting elements required for strong stop acceptor template selection during Moloney murine leukemia virus reverse transcription.
Cis-acting elements required for strong stop acceptor template selection during Moloney murine leukemia virus reverse transcription.
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莫洛尼鼠白血病病毒逆转录过程中强终止受体模板选择所需的顺式作用元件。
DOI：
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发表时间：
1998
影响因子：
5.6
通讯作者：
A. Telesnitsky
中科院分区：
文献类型：
--
作者：
Robert S. Topping;M. Demoitié;Nam;A. Telesnitsky
模板转换在正常的逆转录病毒DNA合成过程中是必需的，并参与逆转录病毒的遗传重组。研究了莫洛尼鼠白血病病毒逆转录过程中的第一个强终止模板开关，以检查如何选择模板开关受体模板。逆转录病毒载体与特定的改变，在其模板转换受体区域的构建，和DNA产物模板，这些载体在一个单一的复制周期进行了分析。结果表明，使引物链3'端和受体模板之间的互补性最大化不是确定强终止模板转换位点的最重要因素。相反，观察到优先转移到U3/R接合处，引物末端和模板链之间的互补性少至一个连续碱基对，足以指导模板转换到U3/R接合处。这些研究结果表明，与流行的教条相反，一个碱基配对独立的机制在逆转录病毒强终止模板切换到U3/R连接的特定指导下发挥作用。模板转换位点3'的某些模板改变至少与引物末端错配一样破坏受体模板的使用，表明模板结构或引物链内部序列是受体模板选择的重要决定因素。我们将讨论这些发现对逆转录病毒DNA合成和同源重组机制的影响。
Template switching is required during normal retroviral DNA synthesis and is involved in retroviral genetic recombination. The first strong stop template switch during Moloney murine leukemia virus reverse transcription was studied to examine how template switch acceptor templates are selected. Retroviral vectors with specific alterations in their template switch acceptor regions were constructed, and DNA products templated by these vectors during a single replication cycle were analyzed. The results indicated that maximizing complementarity between the primer strand 3' end and the acceptor template was not the most significant factor in determining a strong stop template switch site. Instead, preferential transfer to the U3/R junction was observed, with as little as one contiguous base-pair of complementarity between the primer terminus and the template strand sufficient to direct template switching to the U3/R junction. These findings suggest that, in contrast to prevailing dogma, a base-pairing-independent mechanism functions in the specific guidance of retroviral strong stop template switch to the U3/R junction. Certain template alterations 3' of the template switch site were at least as disruptive to acceptor template use as was primer-terminal mismatch, suggesting that template structure or primer strand-internal sequences are important determinants of acceptor template selection. We discuss the implications of these findings for the mechanisms of retroviral DNA synthesis and homologous recombination.
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影响因子：
2.9
作者：
J. A. Peliska;Shankar Balasubramanian;D. Giedroc;Stephen J. Benkovic
通讯作者：
J. A. Peliska;Shankar Balasubramanian;D. Giedroc;Stephen J. Benkovic
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Creighton,S;Huang,MM;Cai,H;Arnheim,N;Goodman,MF
通讯作者：
Goodman,MF
DOI：
10.1073/pnas.86.21.8343
发表时间：
1989-11-01
影响因子：
11.1
作者：
PERRINO, FW;PRESTON, BD;LOEB, LA
通讯作者：
LOEB, LA
DOI：
10.1016/s0021-9258(19)51068-7
发表时间：
1994-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Zinnen;J. Hsieh;Paul ModrichS
通讯作者：
S. Zinnen;J. Hsieh;Paul ModrichS
逆转录病毒逆转录酶测定。
影响因子：
--
作者：
Telesnitsky,A;Blain,S;Goff,SP
通讯作者：
Goff,SP