Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna).
Regulation and dysregulation of vitellogenin mRNA accumulation in daphnids (Daphnia magna).
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DOI：
10.1016/j.aquatox.2010.11.006
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发表时间：
2011-01-25
影响因子：
4.5
通讯作者：
LeBlanc, Gerald A.
中科院分区：
文献类型：
--
作者：
Hannas, Bethany R.;Wang, Ying H.;Thomson, Susanne;Kwon, Gwijun;Li, Hong;LeBlanc, Gerald A.
卵生脊椎动物卵黄蛋白原的诱导已成为环境中雌激素类化学物质暴露的金标准生物标志物。这种雌激素暴露的生物标志物也被用于节肢动物，然而，很少有人知道的因素，调节这些生物体中的卵黄蛋白原的表达。我们调查的卵黄蛋白原基因Vtg 2在水蚤（大型蚤）暴露于各种化学品的mRNA产物的积累的变化。我们进一步评估了参与调节卵黄蛋白原表达的激素因素，这可能是目标的外源性化学物质。Vtg 2基因的表达对暴露于各种化学物质具有高度响应性，其表达范围跨越约四个数量级。引起最大诱导的化学品是胡椒基丁醚、三氯乙烯、4-壬基苯酚、镉和氯仿。其中，只有4-壬基酚被认为是雌激素。暴露于几种化学物质也抑制Vtg 2 mRNA水平，高达100倍。抑制性化学物质包括醋酸环丙孕酮、丙酮、三氯生和阿特拉津。暴露于雌激素己烯雌酚和双酚A对卵黄蛋白原mRNA水平几乎没有影响，进一步证实这些基因不是由雌激素暴露诱导的。暴露于有效的蜕皮类固醇20-羟基蜕皮激素和ponasterone A揭示，Vtg 2受到这些激素的强烈抑制控制。Vtg 2 mRNA水平没有显着影响暴露于几个juvenoid激素。结果表明，蜕皮甾体是卵黄蛋白原基因表达的抑制剂，卵黄蛋白原mRNA水平可以升高或抑制水蚤的异生物素引起的抗蜕皮甾体或蜕皮甾体活性，分别。重要的是，水蚤Vtg 2不升高，雌激素活性的反应。
The induction of vitellogenin in oviparous vertebrates has become the gold standard biomarker of exposure to estrogenic chemicals in the environment. This biomarker of estrogen exposure also has been used in arthropods, however, little is known of the factors that regulate the expression of vitellogenin in these organisms. We investigated changes in accumulation of mRNA products of the vitellogenin gene Vtg2 in daphnids (Daphnia magna) exposed to a diverse array of chemicals. We further evaluated the involvement of hormonal factors in the regulation of vitellogenin expression that may be targets of xenobiotic chemicals. Expression of the Vtg2 gene was highly responsive to exposure to various chemicals with an expression range spanning approximately four orders of magnitude. Chemicals causing the greatest induction were piperonyl butoxide, chlordane, 4-nonylphenol, cadmium, and chloroform. Among these, only 4-nonylphenol is recognized to be estrogenic. Exposure to several chemicals also suppressed Vtg2 mRNA levels, as much as 100-fold. Suppressive chemicals included cyproterone acetate, acetone, triclosan, and atrazine. Exposure to the estrogens diethylstilbestrol and bisphenol A had little effect on vitellogenin mRNA levels further substantiating that these genes are not induced by estrogen exposure. Exposure to the potent ecdysteroids 20-hydroxyecdysone and ponasterone A revealed that Vtg2 was subject to strong suppressive control by these hormones. Vtg2 mRNA levels were not significantly affected from exposure to several juvenoid hormones. Results indicate that ecdysteroids are suppressors of vitellogenin gene expression and that vitellogenin mRNA levels can be elevated or suppressed in daphnids by xenobiotics that elicit antiecdysteroidal or ecdysteroidal activity, respectively. Importantly, daphnid Vtg2 is not elevated in response to estrogenic activity.
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DOI：
10.1080/15287390701551399
发表时间：
2007-01-01
影响因子：
2.6
作者：
Burke, Angela S.;Redeker, Kelly;Hinson, Jack A.
通讯作者：
Hinson, Jack A.
影响因子：
10.4
作者：
HEPPELL, SA;DENSLOW, ND;SULLIVAN, CV
通讯作者：
SULLIVAN, CV
DOI：
10.1668/0003-1569(2001)041
发表时间：
2001-04-01
期刊：
AMERICAN ZOOLOGIST
影响因子：
--
作者：
Kramer, DL;McLaughlin, RL
通讯作者：
McLaughlin, RL
影响因子：
4.8
作者：
Kimura, S;Tokishita, S;Yamagata, H
通讯作者：
Yamagata, H
影响因子：
3.6
作者：
Honer, C;Nam, K;Schumacher, C
通讯作者：
Schumacher, C