Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells.
Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells.
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DOI：
10.1111/j.1582-4934.2007.00103.x
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发表时间：
2008-01
影响因子：
5.3
通讯作者：
Rychly J
中科院分区：
文献类型：
--
作者：
Müller P;Bulnheim U;Diener A;Lüthen F;Teller M;Klinkenberg ED;Neumann HG;Nebe B;Liebold A;Steinhoff G;Rychly J
尽管体内研究揭示了支架与成骨祖细胞一起植入后骨再生的有希望的结果，但基本问题仍然是材料表面如何控制间充质干细胞（MSC）的生物学。我们使用源自骨髓的人 MSC，研究了磷酸钙表面的成骨分化。在成骨分化培养基中，MSC 在 14 天内在羟基磷灰石和 BONITmatrix®（一种可降解干凝胶复合材料）上分化为成骨细胞。与组织培养塑料上的细胞相比，使用实时 RTPCR 技术发现细胞具有更高的碱性磷酸酶 (ALP) 活性，并且 I 型胶原蛋白和骨钙素的 RNA 表达增加。为了测试材料表面特征是否能够单独刺激成骨分化，在不含可溶性成骨分化添加剂的扩张培养基中的材料上培养MSC。事实上，在没有成骨分化添加剂的磷酸钙上的细胞发育成成骨细胞，如 ALP 活性和成骨基因表达增加所示，而在组织培养塑料上的情况并非如此。因为我们推断磷酸钙表面对成骨的刺激作用取决于细胞与细胞外基质相互作用的改变，所以我们使用用 GFP 标记的纽蛋白转染的细胞研究了粘着斑的动态行为。与组织培养塑料上的细胞相比，在 BONITmatrix® 上观察到粘着斑的移动性增加。总之，在培养基中缺乏成骨分化补充剂的情况下，磷酸钙表面能够驱动 MSC 分化为成骨细胞。磷酸钙表面粘着斑动态行为的改变可能与促进成骨分化的分子机制有关。
Although studies in vivo revealed promising results in bone regeneration after implantation of scaffolds together with osteogenic progenitor cells, basic questions remain how material surfaces control the biology of mesenchymal stem cells (MSC). We used human MSC derived from bone marrow and studied the osteogenic differentiation on calcium phosphate surfaces. In osteogenic differentiation medium MSC differentiated to osteoblasts on hydroxyapatite and BONITmatrix®, a degradable xerogel composite, within 14 days. Cells revealed a higher alkaline phosphatase (ALP) activity and increased RNA expression of collagen I and osteocalcin using real-time RTPCR compared with cells on tissue culture plastic. To test whether material surface characteristics alone are able to stimulate osteogenic differentiation, MSC were cultured on the materials in expansion medium without soluble additives for osteogenic differentiation. Indeed, cells on calcium phosphate without osteogenic differentiation additives developed to osteoblasts as shown by increased ALP activity and expression of osteogenic genes, which was not the case on tissue culture plastic. Because we reasoned that the stimulating effect on osteogenesis by calcium phosphate surfaces depends on an altered cell–extracellular matrix interaction we studied the dynamic behaviour of focal adhesions using cells transfected with GFP labelled vinculin. On BONITmatrix®, an increased mobility of focal adhesions was observed compared with cells on tissue culture plastic. In conclusion, calcium phosphate surfaces are able to drive MSC to osteoblasts in the absence of osteogenic differentiation supplements in the medium. An altered dynamic behaviour of focal adhesions on calcium phosphate surfaces might be involved in the molecular mechanisms which promote osteogenic differentiation.
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影响因子：
14
作者：
Curran, Judith M.;Chen, Rui;Hunt, John A.
通讯作者：
Hunt, John A.
影响因子：
4.8
作者：
Jadlowiec, J;Koch, H;Sfeir, C
通讯作者：
Sfeir, C
影响因子：
3.3
作者：
Klees, RF;Salasznyk, RM;Plopper, GE
通讯作者：
Plopper, GE
影响因子：
2.9
作者：
Franceschi, RT;Xiao, GZ;Reith, E
通讯作者：
Reith, E
DOI：
10.1007/b99997
发表时间：
2005-01-01
期刊：
REGENERATIVE MEDICINE II: CLINICAL AND PRECLINICAL APPLICATIONS
影响因子：
--
作者：
Mistry, AS;Mikos, AG
通讯作者：
Mikos, AG