Bacterial collagen-like proteins that form triple-helical structures.
Bacterial collagen-like proteins that form triple-helical structures.
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DOI：
10.1016/j.jsb.2014.01.003
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发表时间：
2014-06
影响因子：
3
通讯作者：
Brodsky, Barbara
中科院分区：
文献类型：
--
作者：
Yu, Zhuoxin;An, Bo;Ramshaw, John A. M.;Brodsky, Barbara
在过去的十年中，已经在细菌中鉴定了大量的胶原样蛋白，主要是从基因组数据库的分析。这些细菌胶原蛋白共享动物胶原蛋白的独特的Gly-Xaa-Yaa重复氨基酸序列，这是其独特的三螺旋结构的基础。许多细菌胶原蛋白已在大肠杆菌中表达。大肠杆菌，它们都采用三螺旋构象。与动物胶原蛋白不同，这些细菌蛋白质不含后修饰的氨基酸羟脯氨酸，羟脯氨酸已知可稳定三螺旋结构并可促进自组装。尽管不存在胶原羟基化，但所研究的细菌胶原的三螺旋结构表现出35-39 °C的高热稳定性，接近哺乳动物胶原的热稳定性。这些细菌胶原蛋白容易通过重组方法以原始氨基酸序列或遗传操作序列大量产生。这种新的重组、易于修饰的胶原蛋白家族可以为研究动物胶原蛋白中的结构和功能基序提供一种新的系统，也可以形成具有设计结构特性和功能的新生物医学材料的基础。
A large number of collagen-like proteins have been identified in bacteria during the past ten years, principally from analysis of genome databases. These bacterial collagens share the distinctive Gly-Xaa-Yaa repeating amino acid sequence of animal collagens which underlies their unique triple-helical structure. A number of the bacterial collagens have been expressed in E. coli, and they all adopt a triple-helix conformation. Unlike animal collagens, these bacterial proteins do not contain the post-translationally modified amino acid, hydroxyproline, which is known to stabilize the triple-helix structure and may promote self-assembly. Despite the absence of collagen hydroxylation, the triple-helix structures of the bacterial collagens studied exhibit a high thermal stability of 35–39 °C, close to that seen for mammalian collagens. These bacterial collagens are readily produced in large quantities by recombinant methods, either in the original amino acid sequence or in genetically manipulated sequences. This new family of recombinant, easy to modify collagens could provide a novel system for investigating structural and functional motifs in animal collagens and could also form the basis of new biomedical materials with designed structural properties and functions.
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影响因子：
2.9
作者：
Culpepper, Megen A.;Scott, Emily E.;Limburg, Julian
通讯作者：
Limburg, Julian
影响因子：
4.8
作者：
Daubenspeck, JM;Zeng, HD;Turnbough, CL
通讯作者：
Turnbough, CL
影响因子：
56.9
作者：
BELLA, J;EATON, M;BERMAN, HM
通讯作者：
BERMAN, HM
DOI：
10.4161/bbug.1.2.10484
发表时间：
2010-03-01
期刊：
Bioengineered bugs
影响因子：
--
作者：
Adrio, Jose-Luis;Demain, Arnold L
通讯作者：
Demain, Arnold L
影响因子：
6.2
作者：
Browning, Mary Beth;Russell, Brooke;Cosgriff-Hernandez, Elizabeth M.
通讯作者：
Cosgriff-Hernandez, Elizabeth M.