Replication and conjugative mobilization of broad host-range IncQ plasmids.

Replication and conjugative mobilization of broad host-range IncQ plasmids.
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广泛的宿主范围INCQ质粒的复制和共轭动员。

DOI:
10.1016/j.plasmid.2009.05.001
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发表时间:
2009-09
期刊:
影响因子:
2.6
通讯作者:
Meyer R
Meyer R
中科院分区:
生物学3区
文献类型:
--
作者:
Meyer R

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IncQ质粒比细菌中任何其他已知的复制元件具有更广泛的宿主范围。这些质粒的复制和接合动员的研究,主要集中在几乎相同的RSF1010和R1162,总结了为了了解如何实现这一广泛的主机范围。IncQ质粒的几个显著特征从这些研究中显现:(1)复制的起始,涉及起始点和专用引发酶的DNA非依赖性激活,是严格不依赖于宿主的。(2)质粒可以通过多种不同的IV型转运蛋白结合动员,包括那些参与分泌致病相关蛋白的转运蛋白。(3)稳定性是由高拷贝数和调节基因表达的组合来保证的,以减少代谢负荷。
The IncQ plasmids have a broader host-range than any other known replicating element in bacteria. Studies on the replication and conjugative mobilization of these plasmids, which have mostly been focused on the nearly identical RSF1010 and R1162, are summarized with a view to understanding how this broad host-range is achieved. Several significant features of IncQ plasmids emerge from these studies: (1) initiation of replication, involving DnaA-independent activation of the origin and a dedicated primase, is strictly host-independent. (2) The plasmids can be conjugatively mobilized by a variety of different type IV transporters, including those engaged in the secretion of proteins involved in pathogenesis. (3) Stability is insured by a combination of high copy-number and modulated gene expression to reduce metabolic load.
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