In vitro and in vivo induction of heme oxygenase‐1 in rat glial cells: Possible involvement of nitric oxide production from inducible nitric oxide synthase
In vitro and in vivo induction of heme oxygenase‐1 in rat glial cells: Possible involvement of nitric oxide production from inducible nitric oxide synthase
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大鼠神经胶质细胞中血红素加氧酶-1 的体外和体内诱导：可能涉及诱导型一氧化氮合酶产生一氧化氮
作者：
Y. Kitamura;Muneki Furukawa;Y. Matsuoka;I. Tooyama;H. Kimura;Yasuyuki Nomura;T. Taniguchi
为了确定血红素加氧酶-1（HO-1）蛋白是否由大鼠神经胶质细胞培养物中的内源性一氧化氮（NO）诱导，我们检测了脂多糖（LPS）、干扰素-γ（IFN-γ）和NO供体如S-亚硝基-N-乙酰青霉胺（SNAP）在混合神经胶质细胞和体内大鼠海马中的作用。在培养的神经胶质细胞中，LPS处理6 h后诱导130 kd诱导型NO合酶（iNOS）表达，12 h后诱导NO2−积累和33 kd HO-1蛋白水平升高。此外，用SNAP处理6小时后诱导HO-1表达。虽然NOS抑制剂如NG-硝基-L-精氨酸（NNA）和NG-甲基-L-精氨酸不改变LPS诱导的iNOS表达，但这些抑制剂抑制NO2-积累和HO-1的增强。免疫细胞化学显示，LPS处理24 h主要在阿米巴样小胶质细胞中诱导iNOS免疫反应，而该处理在小胶质细胞和星形胶质细胞中均诱导HO-1免疫反应。在体内大鼠海马中，24 h后显微注射LPS + IFN-γ或SNAP也诱导反应性小胶质细胞和星形胶质细胞中的HO-1免疫反应性。此外，腹腔内注射NNA抑制了由LPS + IFN-γ诱导的HO-1免疫反应性。这些结果表明，小胶质细胞中iNOS的内源性NO产生导致体外和大鼠脑中小胶质细胞和星形胶质细胞中HO-1蛋白的自分泌和旁分泌诱导。GLIA 22：138-148，1998.© 1998 Wiley利斯公司。
To determine whether heme oxygenase‐1 (HO‐1) protein is induced by endogenous nitric oxide (NO) in rat glial cultures, we examined the effects of lipopolysaccharide (LPS), interferon‐γ (IFN‐γ), and NO donors such as S‐nitroso‐N‐acetylpenicillamine (SNAP), in mixed glial cells and in vivo rat hippocampus. In cultured glial cells, treatment with LPS induced the expression of 130‐kd inducible NO synthase (iNOS) after 6 h, and NO2−accumulation and enhancement of the protein level of 33‐kd HO‐1 after 12 h. In addition, treatment with SNAP induced HO‐1 expression after 6 h. Although NOS inhibitors such as NG‐nitro‐L‐arginine (NNA) and NG‐methyl‐L‐arginine did not change LPS‐induced iNOS expression, these inhibitors suppressed both NO2− accumulation and the enhancement of HO‐1. Immunocytochemistry showed that treatment with LPS for 24 h induced iNOS immunoreactivity predominantly in ameboid microglia, while this treatment induced HO‐1‐immunoreactivity in both microglia and astrocytes. In in vivo rat hippocampus, microinjection of LPS plus IFN‐γ, or SNAP after 24 h also induced HO‐1 immunoreactivity in reactive microglia and astrocytes. In addition, intraperitoneal administration of NNA inhibited HO‐1 immunoreactivity induced by the microinjection of LPS plus IFN‐γ. These results suggest that endogenous NO production by iNOS in microglia causes autocrine and paracrine induction of HO‐1 protein in microglia and astrocytes in vitro and in rat brain. GLIA 22:138–148, 1998.© 1998 Wiley‐Liss, Inc.
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影响因子：
2.9
作者：
GREEN, LC;WAGNER, DA;TANNENBAUM, SR
通讯作者：
TANNENBAUM, SR
DOI：
10.1016/s0021-9258(19)39141-0
发表时间：
1990-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mitch O. Rotenberg;M. Maines
通讯作者：
Mitch O. Rotenberg;M. Maines
人血红素加氧酶-2：全长 cDNA 的表征和表达，以及表明两个 HO-2 转录物可能因聚腺苷酸化信号的选择而异的证据。
影响因子：
3.9
作者：
McCoubreyJr,WK;Ewing,JF;Maines,MD
通讯作者：
Maines,MD
DOI：
10.1073/pnas.93.19.10393
发表时间：
1996-09-17
影响因子：
11.1
作者：
Lee, PJ;Alam, J;Choi, AMK
通讯作者：
Choi, AMK
DOI：
10.1016/0169-328x(95)00315-j
发表时间：
1996-04-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Nimura, T;Weinstein, PR;Sharp, FR
通讯作者：
Sharp, FR