The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.
The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.
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DOI：
10.1002/mnfr.202200090
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发表时间：
2022-10
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
人类母乳含有多种细胞类型，对婴儿免疫和发育具有潜在作用。与定义婴儿乳细胞的目的相关的一个挑战是对消化对细胞命运的影响的理解不足。这项研究首次证明了小鼠胃消化后发生乳细胞死亡。然后使用流式细胞术和RT-PCR来了解人乳细胞死亡的机制，并在模拟胃消化前后定量活细胞类型。这项研究发现，在模拟胃液中消化30分钟将细胞活力从72%降低到27%，大多数细胞死亡是由酸性pH引起的。牛奶中的非细胞成分只能提供轻微的保护，防止胃酸引起的细胞死亡。胃消化并不选择特定的弹性细胞群体存活-大多数细胞类型在胃环境中以相同的比例死亡。总之，这些结果为了解婴儿肠道及其他部位人类母乳细胞的命运提供了基础。母乳中含有母体细胞，在婴儿发育中具有潜在作用。这项研究表明，大多数来自人乳的细胞在模拟胃液中消化30分钟后通过凋亡而死亡，而没有选择弹性细胞类型。这些结果为了解乳细胞在肠道内外的命运提供了基础。
Human breast milk contains a variety of cell types that have potential roles in infant immunity and development. One challenge associates with defining the purpose(s) of milk cells in the infant is a poor understanding of the effect of digestion on cell fate. This study first demonstrates that milk cell death occurs after gastric digestion in mice. Then flow cytometry and RT‐PCR are used to understand the mechanism of human milk cell death and quantify live cell types before and after simulated gastric digestion. This study finds that digestion in simulated gastric fluid for 30 min reduces cell viability from 72% to 27%, with most cell death is caused by the acidic pH. The primary mechanism of cell death is caspase‐mediated apoptosis. The non‐cellular components of milk offer only mild protection against cell death from stomach acid. Gastric digestion does not select for a specific resilient cell population to survive—most cell types die in equal proportions in the gastric environment. Taken together, these results provide a foundation with which to understand the fate of human breast milk cells in the infant's intestine and beyond. Human milk contains maternal cells with potential roles in infant development. This study demonstrates that the majority of cells from human milk die by apoptosis after 30 min of digestion in simulated gastric fluid, without selecting for a resilient cell type. These results provide a foundation to understand the fate of milk cells in the intestine and beyond.
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影响因子：
4.6
作者：
Ball RL;Bajaj P;Whitehead KA
通讯作者：
Whitehead KA
影响因子：
4.4
作者：
Ghosh, Mrinal K.;Nguyen, Virginia;Walker, Ameae M.
通讯作者：
Walker, Ameae M.
影响因子：
5.2
作者：
Hassiotou, Foteini;Beltran, Adriana;Chetwynd, Ellen;Stuebe, Alison M.;Twigger, Alecia-Jane;Metzger, Philipp;Trengove, Naomi;Lai, Ching Tat;Filgueira, Luis;Blancafort, Pilar;Hartmann, Peter E.
通讯作者：
Hartmann, Peter E.
影响因子：
11.2
作者：
Charafe-Jauffret E;Ginestier C;Iovino F;Wicinski J;Cervera N;Finetti P;Hur MH;Diebel ME;Monville F;Dutcher J;Brown M;Viens P;Xerri L;Bertucci F;Stassi G;Dontu G;Birnbaum D;Wicha MS
通讯作者：
Wicha MS
DOI：
10.1136/adc.64.7_spec_no.930
发表时间：
1989-07-01
影响因子：
4.4
作者：
JAIN, L;VIDYASAGAR, D;BLEND, M
通讯作者：
BLEND, M