Studies on the recombination genes of bacteriophage T4: suppression of uvsX and uvsY mutations by uvsW mutations.
Studies on the recombination genes of bacteriophage T4: suppression of uvsX and uvsY mutations by uvsW mutations.
复制标题
噬菌体T4重组基因的研究：uvsW突变抑制uvsX和uvsY突变。
DOI：
10.1093/genetics/115.2.219
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
T. Minagawa
中科院分区：
文献类型：
--
作者：
T. Yonesaki;T. Minagawa
UvsW、uvsX和uvsY基因对于T4的生长是必不可少的，但与重组和修复受损的DNA有关。我们发现42度的大空斑突变体是从小空斑uvsX和uvsY突变体中有效地产生的，并且是含有uvsW新突变的假逆变突变体。利用重构的双突变体，我们证实了uvsW的突变部分增加了uvsX和uvsY突变体的爆发大小和抗紫外线能力。在41度时，uvsW突变完全恢复了由基因uvsX、uvsY和46突变导致的DNA合成停止，但在30度时，它仅部分恢复了基因46突变体的DNA合成，而不能恢复uvsX和uvsY突变体的DNA合成。在41度恢复DNA合成的同时，单链DNA和基因32蛋白的过度生产是平行的。基于这些发现，我们提出了uvsW基因调控单链DNA的产生，并讨论了uvsW突变体的表型及其对某些uvsX和uvsY表型的抑制。用amuvsW突变体感染限制性细胞，发现一种分子量为53,000道尔顿的蛋白质的合成存在缺陷，表明该蛋白质是uvsW基因产物。
Genes uvsW, uvsX and uvsY are dispensable for T4 growth but are implicated in recombination and in the repair of damaged DNA. We found that large-plaque mutants arose efficiently from small-plaque uvsX and uvsY mutants at 42 degrees and were pseudorevertants containing a new mutation in uvsW. Using reconstructed double mutants, we confirmed that a mutation in uvsW partially increases the burst size and UV resistance of uvsX and uvsY mutants. At 41 degrees the uvsW mutation completely restores the arrest in DNA synthesis caused by mutations in genes uvsX, uvsY and 46, but at 30 degrees it only partially restores DNA synthesis in a gene 46 mutant and does not restore DNA synthesis in uvsX and uvsY mutants. Restored DNA synthesis at 41 degrees was paralleled by the overproduction of single-stranded DNA and gene 32 protein. Based on these findings, we propose that the uvsW gene regulates the production of single-stranded DNA and we discuss the phenotype of uvsW mutants and their suppression of some uvsX and uvsY phenotypes. Infection of restrictive cells with am uvsW mutants revealed a defect in the synthesis of a protein of molecular weight 53,000 daltons, suggesting that this protein is the uvsW gene product.
影响因子：
5.6
作者：
M. L. Parker;A. Christensen;A. Boosman;J. Stockard;E. Young;A. Doermann
通讯作者：
M. L. Parker;A. Christensen;A. Boosman;J. Stockard;E. Young;A. Doermann