Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin.
Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin.
复制标题
DOI：
10.1083/jcb.201110076
复制
发表时间：
2012-08-20
期刊：
影响因子：
--
通讯作者：
Wedlich D
中科院分区：
文献类型：
--
作者：
Kraft B;Berger CD;Wallkamm V;Steinbeisser H;Wedlich D
Wnt-11/ zzzzed -7通过捕获原钙粘蛋白PAPC和c -钙粘蛋白形成不同的粘附调节复合物，减少c -钙粘蛋白的横向聚集。在非洲爪蟾原肠胚形成过程中，Wnt-11/平面细胞极性信号通路使正在进行会聚伸展的中胚层细胞极化。这些与侧插行为相关的形状变化需要细胞粘附的动态调节。在本文中，我们报道了Wnt-11/ zzzzled -7 （Fz7）通过不同的Fz7相互作用域与旁轴原钙粘蛋白（PAPC，表示为AMCP）和c -钙粘蛋白（表示为AMCC）形成单独的粘附调节复合物（AMCs）来控制细胞粘附。当PAPC是Wnt-11-Fz7复合体的一部分时，其依赖于Dynamin1和网格蛋白的内化被阻断。Wnt-11对AMCP （Fz7/PAPC）的膜稳定性阻止了C-cadherin聚集，导致细胞粘附降低和细胞分选活性改变。重要的是，Wnt-11不影响c -钙粘蛋白内化；相反，它促进AMCC （Fz7/Cadherin）的形成，与C-cadherin的顺式二聚化相竞争。由于PAPC和C-cadherin不直接相互作用，也不与Fz7形成联合复合体，我们认为Wnt-11触发两种不同复合体AMCC和AMCP的形成，这两种复合体通过阻碍C-cadherin的横向聚集而平行作用，以减少细胞粘附。
Wnt-11/frizzled-7 reduces the lateral clustering of C-cadherin by capturing the protocadherin PAPC and C-cadherin into distinct adhesion-modulating complexes. Wnt-11/planar cell polarity signaling polarizes mesodermal cells undergoing convergent extension during Xenopus laevis gastrulation. These shape changes associated with lateral intercalation behavior require a dynamic modulation of cell adhesion. In this paper, we report that Wnt-11/frizzled-7 (Fz7) controls cell adhesion by forming separate adhesion-modulating complexes (AMCs) with the paraxial protocadherin (PAPC; denoted as AMCP) and C-cadherin (denoted as AMCC) via distinct Fz7 interaction domains. When PAPC was part of a Wnt-11–Fz7 complex, its Dynamin1- and clathrin-dependent internalization was blocked. This membrane stabilization of AMCP (Fz7/PAPC) by Wnt-11 prevented C-cadherin clustering, resulting in reduced cell adhesion and modified cell sorting activity. Importantly, Wnt-11 did not influence C-cadherin internalization; instead, it promoted the formation of AMCC (Fz7/Cadherin), which competed with cis-dimerization of C-cadherin. Because PAPC and C-cadherin did not directly interact and did not form a joint complex with Fz7, we suggest that Wnt-11 triggers the formation of two distinct complexes, AMCC and AMCP, that act in parallel to reduce cell adhesion by hampering lateral clustering of C-cadherin.
登录
查看更多内容
DOI：
10.1083/jcb.135.2.487
发表时间：
1996-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brieher WM;Yap AS;Gumbiner BM
通讯作者：
Gumbiner BM
影响因子：
10.5
作者：
Habas, R;Dawid, IB;He, X
通讯作者：
He, X
突变动力蛋白的诱导特异性阻断内吞涂层囊泡的形成。
影响因子：
7.8
作者：
Damke, H;Baba, T;Warnock, D E;Schmid, S L
通讯作者：
Schmid, S L
影响因子：
5.7
作者：
Harrison, Oliver J.;Jin, Xiangshu;Hong, Soonjin;Bahna, Fabiana;Ahlsen, Goran;Brasch, Julia;Wu, Yinghao;Vendome, Jeremie;Felsovalyi, Klara;Hampton, Cheri M.;Troyanovsky, Regina B.;Ben-Shau, Avinoam;Frank, Joachim;Troyanovsky, Sergey M.;Shapiro, Lawrence;Honig, Barry
通讯作者：
Honig, Barry
钙粘蛋白介导的细胞分选不能由结合或粘附特异性确定。
影响因子：
7.8
作者：
Niessen, Carien M;Gumbiner, Barry M
通讯作者：
Gumbiner, Barry M