Transcriptome-Wide Identification of Differentially Expressed Genes and Long Non-coding RNAs in Aluminum-Treated Rat Hippocampus
Transcriptome-Wide Identification of Differentially Expressed Genes and Long Non-coding RNAs in Aluminum-Treated Rat Hippocampus
复制标题
铝处理大鼠海马差异表达基因和长非编码 RNA 的全转录组鉴定
DOI：
10.1007/s12640-018-9879-1
复制
发表时间：
2018-02
影响因子：
3.7
通讯作者：
Qiao Niu
中科院分区：
文献类型：
--
作者：
Yirong Xu;Huifang Zhang;Baolong Pan;Shuhui Zhang;Shan Wang;Qiao Niu
Aluminum (Al) is an environmental neurotoxicant with a wide exposure, but the molecular mechanism underlying its toxicity remains unclear. We used RNA sequencing (RNA-seq) in the hippocampus of Al-treated rats to identify 96 upregulated and 652 downregulated mRNAs, and 37 dysregulated long non-coding (lnc)RNAs. Gene ontology analysis showed that dysregulated genes were involved in glial cell differentiation, neural transmission, and vesicle trafficking. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed clustering of differentially expressed mRNAs and lncRNA target genes in several pathways, including the “adenosine monophosphate-activated protein kinase signaling pathway,” “extracellular matrix receptor interaction,” “the phosphatidylinositol 3 kinase–protein kinase B signaling pathway,” and “focal adhesion” signaling pathway. RNA-seq results were validated by reverse transcription (RT)-PCR. Additionally, Al induced changes to the number and morphology of glial cells in the hippocampus of rats, as shown by glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) immunochemistry. RT-PCR and western blotting validated the significant increase in expression of glial cell-related genesGFAPandSOX10following Al exposure compared with control rats, consistent with RNA-seq results. Collectively, these results suggest that aberrant mRNAs and lncRNAs respond to Al neurotoxicity, and that glial cell-related genes play important roles in the Al neurotoxicity mechanism. These findings provide the basis for designing targeted approaches for the treatment or prevention of Al-induced neurotoxicity.
登录
查看更多内容
DOI：
10.1186/s12993-015-0054-z
发表时间：
2015-02-18
期刊：
Behavioral and brain functions : BBF
影响因子：
--
作者：
Wang H;Ye M;Yu L;Wang J;Guo Y;Lei W;Yang J
通讯作者：
Yang J
影响因子：
3.4
作者：
Bondy SC
通讯作者：
Bondy SC
阿尔茨海默病模型中长非编码 RNA 的独特海马表达谱
影响因子：
5.1
作者：
Yang B;Xia ZA;Zhong B;Xiong X;Sheng C;Wang Y;Gong W;Cao Y;Wang Z;Peng W
通讯作者：
Peng W
使用 RNA 测序对衰老加速的小鼠 Prone 8 大脑中的长非编码 RNA 进行系统分析
DOI：
10.1038/mtna.2016.57
发表时间：
2016-08-02
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.3
作者：
Rola, Radoslaw;Mizumatsu, Shinichiro;Fike, John R.
通讯作者：
Fike, John R.