Inhibition of Drosophila EGF receptor activation by the secreted protein Argos
Inhibition of Drosophila EGF receptor activation by the secreted protein Argos
复制标题
分泌蛋白 Argos 抑制果蝇 EGF 受体激活
作者：
R. Schweitzer;R. Howes;Richard F. Smith;B. Shilo;M. Freeman
哺乳动物表皮生长因子（EGF）受体（DER）的果蝇同源物1，2是一种受体酪氨酸激酶，参与果蝇发育的许多阶段，包括光感受器决定和翅静脉形成39。其主要活化配体是Spitz蛋白10，11，与哺乳动物TGF-α相似（参考文献12）。Argos是一种分泌性蛋白质，与Spitz一样，含有单个EGF基序1315。它是眼睛中细胞决定的阻遏物，并在其他组织中起作用，包括翼1618。因为Argos在眼睛中具有与DER相反的作用（前者阻断感光细胞决定，后者促进它），我们已经测试了它是否通过阻断DER途径起作用。我们表明，Argos确实抑制这一途径在体内，并发现，在体外，Argos蛋白可以抑制激活的DER斯皮茨。因此，DER途径对细胞的决定受细胞外激活和抑制信号之间的平衡调节。这是受体酪氨酸激酶的细胞外抑制剂的第一个体内实例。
THE Drosophila homologue of the mammalian epidermal growth factor (EGF) receptor (DER)1,2 is a receptor tyrosine kinase involved in many stages of fly development, including photoreceptor determination, and wing-vein formation39. Its primary activating ligand is the Spitz protein10,11, which is similar to mammalian TGF-α (ref. 12). Argos is a secreted protein that, like Spitz, contains a single EGF motif1315. It is a represser of cell determination in the eye, and acts in other tissues, including the wing1618. Because Argos has the opposite effects to DER in the eye (the former blocks photoreceptor determination, the latter promotes it) we have tested whether it acts by blocking the DER pathway. We show that Argos does indeed repress this pathway in vivo and find that, in vitro, Argos protein can inhibit the activation of DER by Spitz. Thus the determination of cells by the DER pathway is regulated by a balance between extracellular activating and inhibiting signals. This is the first in vivo example of an extracellular inhibitor of a receptor tyrosine kinase.
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果蝇胚胎发生过程中多次需要鱼雷 (DER) 受体酪氨酸激酶。
DOI：
10.1242/dev.115.3.853
发表时间：
1992
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Clifford,R;Schüpbach,T
通讯作者：
Schüpbach,T
影响因子：
3.3
作者：
Robert J. Clifford;Trudi Schupbach
通讯作者：
Robert J. Clifford;Trudi Schupbach
影响因子：
10.5
作者：
M. Sturtevant;M. Roark;E. Bier
通讯作者：
M. Sturtevant;M. Roark;E. Bier
果蝇 EGF 受体同系物 (DER) 蛋白的生化特性。
影响因子：
8
作者：
Zak,NB;Shilo,BZ
通讯作者：
Shilo,BZ