A predicted NEDD8 conjugating enzyme gene identified as a Capsicum candidate Rf gene using bulk segregant RNA sequencing.
A predicted NEDD8 conjugating enzyme gene identified as a Capsicum candidate Rf gene using bulk segregant RNA sequencing.
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使用批量分离 RNA 测序将预测的 NEDD8 结合酶基因鉴定为辣椒候选 Rf 基因
DOI：
10.1038/s41438-020-00425-7
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发表时间：
2020
影响因子：
8.7
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Wei B;Bosland PW;Zhang Z;Wang Y;Zhang G;Wang L;Yu J
细胞质雄性不育(CMS)是产生杂种优势的重要工具。育性恢复系(Rf)的伴生系统在分子水平上知之甚少，在生产杂交制种的恢复系方面很有价值。辣椒中RF基因的特性目前尚不清楚。在本研究中，通过批量分离RNA测序(BSR-seq)，鉴定了一个很强的候选RF基因Capana06g002866，它被注释为NEDD8结合酶E2。Capana06g002866全长555bp，编码184个氨基酸，可从不育系8A与恢复系R1杂交的F1植株中克隆，但在不育系8A中未发现。通过qRT-PCR验证，与不育材料相比，Capana06g002866在恢复系中表达上调。在F1植株中，花芽中的相对表达随着发育阶段的延长而增加，而不育系各花蕾中的相对表达水平都很低。这些结果为研究辣椒的Rf基因提供了新的思路，并将对利用CMS系统的其他作物有用。
Cytoplasmic male sterility (CMS) is an important tool for producing F 1 hybrids, which can exhibit heterosis. The companion system, restorer-of-fertility (Rf), is poorly understood at the molecular level and would be valuable in producing restorer lines for hybrid seed production. The identity of the Rf gene in Capsicum (pepper) is currently unclear. In this study, using bulked segregant RNA sequencing (BSR-seq), a strong candidate Rf gene, Capana06g002866, which is annotated as a NEDD8 conjugating enzyme E2, was identified. Capana06g002866 has an ORF of 555 bp in length encoding 184 amino acids; it can be cloned from F 1 plants from the hybridization of the CMS line 8A and restorer line R1 but is not found in CMS line 8A. With qRT-PCR validation, Capana06g002866 was found to be upregulated in restorer accessions compared to sterile accessions. The relative expression in flower buds increased with the developmental stage in F 1 plants, while the expression was very low in all flower bud stages of the CMS lines. These results provide new insights into the Rf gene in pepper and will be useful for other crops utilizing the CMS system.
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影响因子：
4.8
作者：
Chen, HY;Sun, JM;Meistrich, ML
通讯作者：
Meistrich, ML
影响因子：
7
作者：
Hill JT;Demarest BL;Bisgrove BW;Gorsi B;Su YC;Yost HJ
通讯作者：
Yost HJ
影响因子：
5.4
作者：
Akagi, H;Nakamura, A;Fujimura, T
通讯作者：
Fujimura, T
DOI：
10.1073/pnas.1007667108
发表时间：
2011-01-25
影响因子：
11.1
作者：
Fujii, Sota;Bond, Charles S.;Small, Ian D.
通讯作者：
Small, Ian D.
DOI：
10.1073/pnas.102301599
发表时间：
2002-08-06
影响因子：
11.1
作者：
Bentolila, S;Alfonso, AA;Hanson, MR
通讯作者：
Hanson, MR