Autoantibodies to transcription intermediary factor TIF1β associated with dermatomyositis.
Autoantibodies to transcription intermediary factor TIF1β associated with dermatomyositis.
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DOI：
10.1186/ar3802
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发表时间：
2012-04-17
影响因子：
4.9
通讯作者：
Chan EK
中科院分区：
文献类型：
--
作者：
Satoh M;Chan JY;Ross SJ;Li Y;Yamasaki Y;Yamada H;Vazquez-del Mercado M;Petri MH;Jara LJ;Saavedra MA;Cruz-Reyes C;Sobel ES;Reeves WH;Ceribelli A;Chan EK
肌炎特异性自身抗体与独特的临床亚群相关，是多发性肌炎/皮肌炎（PM/DM）的有用生物标志物。一个120 kD的蛋白识别的某些糖尿病患者进行了鉴定，并与此特异性的患者的临床特征进行了表征。通过质谱和免疫学方法对原型血清识别的120 kD蛋白进行纯化和鉴定。通过35 S-甲硫氨酸标记的K562细胞提取物的免疫沉淀，在来自四个国家的2，356名具有不同诊断的患者（包括254名PM/DM）的血清中筛选针对该120 kD蛋白的自身抗体。收集具有该特异性的患者的临床信息。经质谱和免疫组化鉴定，PL-12与120 kD的蛋白质完全融合，为转录中间因子TIF 1 β（TRIM 28）。通过免疫荧光，抗-TIF 1 β阳性显示细斑点的核染色模式。确定了4例抗TIF 1 β病例;均为女性，日本人、非裔美国人、高加索人和墨西哥人各1例。3例被诊断为DM，1例被归类为未分化结缔组织病伴CPK升高，但无明显肌肉症状。该患者还有结肠癌、宫颈鳞状上皮化生和子宫纤维瘤病史。所有病例的肌病均为轻度，1例病例未经治疗即消退。在其他条件下未发现抗TIF 1 β特异性。抗TIF 1 β是一种新的糖尿病自身抗体，与轻度肌病相关。它是否与恶性肿瘤相关，如抗TIF 1 γ或其他独特的特征，将需要在未来的研究中进行评估。
Myositis specific autoantibodies are associated with unique clinical subsets and are useful biomarkers in polymyositis/dermatomyositis (PM/DM). A 120 kD protein recognized by certain patients with DM was identified and clinical features of patients with this specificity were characterized. The 120 kD protein recognized by a prototype serum was purified and identified by mass spectrometry and immunological methods. Autoantibody to this 120 kD protein was screened in sera from 2,356 patients with various diagnoses from four countries, including 254 PM/DM, by immunoprecipitation of 35S-methionine labeled K562 cell extracts. Clinical information of patients with this specificity was collected. The 120 kD protein, which exactly comigrated with PL-12, was identified as transcription intermediary factor TIF1β (TRIM28) by mass spectrometry and validated by immunoassays. By immunofluorescence, anti-TIF1β positivity showed a fine-speckled nuclear staining pattern. Four cases of anti-TIF1β were identified; all are women, one each in a Japanese, African American, Caucasian, and Mexican individual. Three had a diagnosis of DM and one case was classified as having an undifferentiated connective tissue disease with an elevated CPK but without significant muscle symptoms. This individual also had a history of colon cancer, cervical squamous metaplasia and fibroid tumors of the uterus. Myopathy was mild in all cases and resolved without treatment in one case. The anti-TIF1β specificity was not found in other conditions. Anti-TIF1β is a new DM autoantibody associated with a mild form of myopathy. Whether it has an association with malignancy, as in the case of anti-TIF1γ, or other unique features will need to be evaluated in future studies.
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影响因子：
11.2
作者：
Chang PC;Fitzgerald LD;Van Geelen A;Izumiya Y;Ellison TJ;Wang DH;Ann DK;Luciw PA;Kung HJ
通讯作者：
Kung HJ
影响因子：
5.4
作者：
Liao, GL;Huang, J;Hayward, SD
通讯作者：
Hayward, SD
影响因子：
5.9
作者：
TARGOFF, IN;ARNETT, FC
通讯作者：
ARNETT, FC
影响因子：
2.3
作者：
Kang EH;Nakashima R;Mimori T;Kim J;Lee YJ;Lee EB;Song YW
通讯作者：
Song YW
影响因子：
4.4
作者：
Ho, Jiapei;Kong, Jacklyn-Wai-Fun;Lim, Yoon-Pin
通讯作者：
Lim, Yoon-Pin