Deep sequencing of Porphyromonas gingivalis and comparative transcriptome analysis of a LuxS mutant.
Deep sequencing of Porphyromonas gingivalis and comparative transcriptome analysis of a LuxS mutant.
复制标题
DOI：
10.3389/fcimb.2012.00079
复制
发表时间：
2012
影响因子：
5.7
通讯作者：
Lamont RJ
中科院分区：
文献类型：
--
作者：
Hirano T;Beck DA;Demuth DR;Hackett M;Lamont RJ
牙龈卟啉单胞菌是慢性和侵袭性牙周病的主要病因。该生物是一种无糖分解厌氧菌，是口腔各种微环境中混合物种生物膜的组成部分。牙龈卟啉卟啉表达了一系列的毒力因子，对其施加严格的控制。高通量测序技术提供了将功能基因组学与基础生物学联系起来的机会。在这项研究中，我们报告定性和定量的RNA-Seq分析牙龈卟啉卟啉的转录组。我们还将RNA-Seq应用于缺乏ai -2介导的细菌通讯的牙龈卟啉卟啉ΔluxS突变体的转录组。转录组分析证实了菌株ATCC 33277所有预测的orf的表达，包括854个假设的蛋白质，并允许鉴定迄今未知的转录单位。共鉴定出12种非编码rna，包括11种小rna和1种钴胺素核糖开关。在LuxS突变体中，57个基因受到差异调控。将外源合成的4,5-二羟基-2,3-戊二酮（DPD， AI-2前体）添加到ΔluxS突变培养物中，补充了一部分基因的表达，表明LuxS参与牙龈假链球菌AI-2信号通路和非信号通路依赖系统。本研究为今后牙龈卟啉卟啉的病理生理研究提供了重要的数据集，并进一步明确了这种口腔病原体的LuxS调控。
Porphyromonas gingivalis is a major etiological agent in chronic and aggressive forms of periodontal disease. The organism is an asaccharolytic anaerobe and is a constituent of mixed species biofilms in a variety of microenvironments in the oral cavity. P. gingivalis expresses a range of virulence factors over which it exerts tight control. High-throughput sequencing technologies provide the opportunity to relate functional genomics to basic biology. In this study we report qualitative and quantitative RNA-Seq analysis of the transcriptome of P. gingivalis. We have also applied RNA-Seq to the transcriptome of a ΔluxS mutant of P. gingivalis deficient in AI-2-mediated bacterial communication. The transcriptome analysis confirmed the expression of all predicted ORFs for strain ATCC 33277, including 854 hypothetical proteins, and allowed the identification of hitherto unknown transcriptional units. Twelve non-coding RNAs were identified, including 11 small RNAs and one cobalamin riboswitch. Fifty-seven genes were differentially regulated in the LuxS mutant. Addition of exogenous synthetic 4,5-dihydroxy-2,3-pentanedione (DPD, AI-2 precursor) to the ΔluxS mutant culture complemented expression of a subset of genes, indicating that LuxS is involved in both AI-2 signaling and non-signaling dependent systems in P. gingivalis. This work provides an important dataset for future study of P. gingivalis pathophysiology and further defines the LuxS regulon in this oral pathogen.
登录
查看更多内容
影响因子：
3.2
作者：
Hovik, Hedda;Yu, Wen-Han;Chen, Tsute
通讯作者：
Chen, Tsute
影响因子：
3.1
作者：
Fong, KP;Gao, L;Demuth, DR
通讯作者：
Demuth, DR
影响因子：
4.8
作者：
Bahekar, Amol Ashok;Singh, Sarabjeet;Arora, Rohit
通讯作者：
Arora, Rohit
影响因子：
3.2
作者：
Doherty, N;Holden, MTG;Winzer, K
通讯作者：
Winzer, K
影响因子：
3.6
作者：
Duran-Pinedo, Ana E.;Nishikawa, Kiyoshi;Duncan, Margaret J.
通讯作者：
Duncan, Margaret J.