Helminth protein vaccine induced follicular T helper cell for enhancement of humoral immunity against Schistosoma japonicum.
Helminth protein vaccine induced follicular T helper cell for enhancement of humoral immunity against Schistosoma japonicum.
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蠕虫蛋白疫苗诱导滤泡 T 辅助细胞增强抗日本血吸虫体液免疫
DOI：
10.1155/2013/798164
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发表时间：
2013
影响因子：
--
通讯作者：
Kang Y
中科院分区：
文献类型：
--
作者：
Zhang J;Gao W;Guo Q;Huang B;Wang B;Xia G;Kang Y
蛋白质疫苗与佐剂的结合已被广泛用于诱导针对包括寄生虫在内的分子靶标的免疫应答，特别是体液免疫应答。滤泡辅助性T细胞（Follicular T helper，Tfh）是B细胞辅助免疫的专门提供者，然而，Tfh细胞在蛋白质疫苗中的诱导作用却鲜有研究。在这里，我们报告，日本血吸虫重组蛋白（SjGST-32）与他克莫司（FK 506）联合增强Tfh细胞的诱导，其表达典型标志物CXCR 5，BCL 6和IL-21，并增强BALB/c小鼠的体液免疫应答。此外，免疫小鼠的生发中心（GC）B细胞和记忆B细胞上IL-21 R的表达增加，表明诱导的Tfh细胞产生的IL-21与IL-21 R相互作用，激活B细胞，维持长期的体液免疫。结果提示，蠕虫蛋白疫苗联合FK 506诱导Tfh细胞产生体液免疫应答，诱导长期体液免疫。
Protein vaccines combined with adjuvants have been widely used to induce immune responses, especially the humoral immune response, against molecular targets including parasites. Follicular T helper (Tfh) cells are the specialized providers of B-cell help, however, the induction of Tfh cells in protein vaccination has been rarely studied. Here, we report that the Schistosoma japonicum recombinant protein (SjGST-32) combined with tacrolimus (FK506) augmented the induction of Tfh cells, which expressed the canonical markers CXCR5, BCL6, and IL-21, and enhanced the humoral immune responses in BALB/c mice. Furthermore, the expression of IL-21R on germinal center (GC) B cells and memory B cells increased in immunized mice, which indicated that IL-21 from the induced Tfh cells interacted with IL-21R for activation of B cells and maintenance of long-lived humoral immunity. Our results suggest that helminth protein vaccine combined with FK506 induces Tfh cell for stimulating humoral immune responses and inducing long-lived humoral immunity.
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影响因子：
64.8
作者：
OKEEFE, SJ;TAMURA, J;ONEILL, EA
通讯作者：
ONEILL, EA
DNA疫苗与左旋咪唑佐剂免疫小鼠对日本血吸虫感染的保护作用
影响因子：
5.5
作者：
Wang, Xiao;Jin, Huali;Wang, Bin
通讯作者：
Wang, Bin
影响因子：
3.1
作者：
Leenstra, T;Acosta, LP;Kurtis, JD
通讯作者：
Kurtis, JD
影响因子：
4.4
作者：
Haynes, Nicole M.;Allen, Christopher D. C.;Cyster, Jason G.
通讯作者：
Cyster, Jason G.
DOI：
10.1084/jem.20090313
发表时间：
2009-05-11
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
King IL;Mohrs M
通讯作者：
Mohrs M