Delivery of mGluR5 siRNAs by Iron Oxide Nanocages by Alternating Magnetic Fields for Blocking Proliferation of Metastatic Osteosarcoma Cells.
Delivery of mGluR5 siRNAs by Iron Oxide Nanocages by Alternating Magnetic Fields for Blocking Proliferation of Metastatic Osteosarcoma Cells.
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DOI：
10.3390/ijms23147944
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发表时间：
2022-07-19
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
虽然骨肉瘤是最常见的原发恶性骨肿瘤，但在过去的三十年里，化疗药物和治疗未能提高五年生存率。我们先前证明了5型代谢性谷氨酸受体mGluR5是骨肉瘤转移细胞增殖所必需的。在这项工作中，我们使用超顺磁性氧化铁纳米笼(IO-Nanocage)作为载体，在体外释放mGluR5 siRNA，并应用交变磁场(AMFS)来促进mGluR5 siRNAs的释放。我们观察了当mGluR5在人和小鼠骨肉瘤细胞系中沉默表达时的功能结果。结果表明，IO-纳米笼成功地携带了mGluR5 siRNAs，AMFS促进了mGluR5 siRNAs的释放，导致mGluR5沉默。此外，我们观察到，当mGluR5在细胞中的表达被沉默时，人和小鼠骨肉瘤细胞的增殖都显著下降。这种新型的磁性siRNA传递方法能够在AMFS下显著沉默骨肉瘤细胞系mGluR5的表达，我们的研究结果表明该方法可以进一步应用于癌症治疗的临床应用。
Although osteosarcoma is the most common primary malignant bone tumor, chemotherapeutic drugs and treatment have failed to increase the five-year survival rate over the last three decades. We previously demonstrated that type 5 metabotropic glutamate receptor, mGluR5, is required to proliferate metastatic osteosarcoma cells. In this work, we delivered mGluR5 siRNAs in vitro using superparamagnetic iron oxide nanocages (IO-nanocages) as delivery vehicles and applied alternating magnetic fields (AMFs) to improve mGluR5 siRNAs release. We observed functional outcomes when mGluR5 expression is silenced in human and mouse osteosarcoma cell lines. The results elucidated that the mGluR5 siRNAs were successfully delivered by IO-nanocages and their release was enhanced by AMFs, leading to mGluR5 silencing. Moreover, we observed that the proliferation of both human and mouse osteosarcoma cells decreased significantly when mGluR5 expression was silenced in the cells. This novel magnetic siRNA delivery methodology was capable of silencing mGluR5 expression significantly in osteosarcoma cell lines under the AMFs, and our data suggested that this method can be further used in future clinical applications in cancer therapy.
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影响因子：
3.7
作者：
Liao S;Ruiz Y;Gulzar H;Yelskaya Z;Ait Taouit L;Houssou M;Jaikaran T;Schvarts Y;Kozlitina K;Basu-Roy U;Mansukhani A;Mahajan SS
通讯作者：
Mahajan SS
影响因子：
46.2
作者：
Behzadi S;Serpooshan V;Tao W;Hamaly MA;Alkawareek MY;Dreaden EC;Brown D;Alkilany AM;Farokhzad OC;Mahmoudi M
通讯作者：
Mahmoudi M
影响因子：
46.2
作者：
Elsabahy M;Wooley KL
通讯作者：
Wooley KL
DOI：
10.1073/pnas.1107304108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Choi, Kyu Yeong;Chang, Kai;Roche, Katherine W.
通讯作者：
Roche, Katherine W.
DOI：
10.1002/ar.23051
发表时间：
2015-02-01
影响因子：
2
作者：
Cheng Teng Ng;Tang, Florence Mei Ai;Bay, Boon Huat
通讯作者：
Bay, Boon Huat