Hypoxia promotes erythroid differentiation through the development of progenitors and proerythroblasts.
Hypoxia promotes erythroid differentiation through the development of progenitors and proerythroblasts.
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DOI：
10.1016/j.exphem.2021.02.012
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发表时间：
2021-05
影响因子：
2.6
通讯作者：
Sharma S
中科院分区：
文献类型：
--
作者：
Bapat A;Schippel N;Shi X;Jasbi P;Gu H;Kala M;Sertil A;Sharma S
Oxygen is a critical noncellular component of the bone marrow microenvironment that plays an important role in the development of hematopoietic cell lineages. In this study, we investigated the impact of low oxygen (hypoxia) on ex vivo myeloerythroid differentiation of human cord blood-derived CD34+ hematopoietic stem and progenitor cells. We characterized the culture conditions to demonstrate that low oxygen inhibits cell proliferation and causes a metabolic shift in the stem and progenitor populations. We found that hypoxia promotes erythroid differentiation by supporting the development of progenitor populations. Hypoxia also increases the megakaryoerythroid potential of the common myeloid progenitors and the erythroid potential of megakaryoerythroid progenitors and significantly accelerates maturation of erythroid cells. Specifically, we determined that hypoxia promotes the loss of CD71 and the appearance of the erythroid markers CD235a and CD239. Further, evaluation of erythroid populations revealed a hypoxia-induced increase in proerythroblasts and in enucleation of CD235a+ cells. These results reveal the extensive role of hypoxia at multiple steps during erythroid development. Overall, our work establishes a valuable model for further investigations into the relationship between erythroid progenitors and/or erythroblast populations and their hypoxic microenvironment.
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影响因子：
50.3
作者：
Carroll PA;Diolaiti D;McFerrin L;Gu H;Djukovic D;Du J;Cheng PF;Anderson S;Ulrich M;Hurley JB;Raftery D;Ayer DE;Eisenman RN
通讯作者：
Eisenman RN
影响因子：
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作者：
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--
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影响因子：
16.6
作者：
Gu, Haiwei;Carroll, Patrick A.;Raftery, Daniel
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Raftery, Daniel
通过减轻氧休克来增强造血干细胞移植功效。
影响因子：
64.5
作者：
Mantel CR;O'Leary HA;Chitteti BR;Huang X;Cooper S;Hangoc G;Brustovetsky N;Srour EF;Lee MR;Messina-Graham S;Haas DM;Falah N;Kapur R;Pelus LM;Bardeesy N;Fitamant J;Ivan M;Kim KS;Broxmeyer HE
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Broxmeyer HE
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5.6
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Hammoud, Mohammad;Vlaski, Marija;Ivanovic, Zoran
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