Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells: Current Controversies.
Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells: Current Controversies.
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DOI：
10.3389/fphys.2021.642812
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发表时间：
2021
影响因子：
4
通讯作者：
Lis R
中科院分区：
文献类型：
--
作者：
Lu TM;Barcia Durán JG;Houghton S;Rafii S;Redmond D;Lis R
脑微血管内皮细胞 (BMEC) 具有独特的特性，这些特性对于血脑屏障 (BBB) 的许多功能至关重要，包括维持大脑稳态以及调节大脑与免疫系统之间的相互作用。已经描述了从人类多能干细胞来源（iBMEC）产生纯的推定脑微血管内皮细胞群体，以满足体外对可靠且可重复的脑内皮细胞的需求。人类多能干细胞（hPSC），无论是胚胎的还是诱导的，都可以分化成大量的特化细胞，以研究发育和疾病模型。这些 hPSC 衍生的 iBMEC 显示出内皮样特性，例如管形成和低密度脂蛋白摄取、高跨内皮电阻 (TEER) 和屏障样外排转运蛋白活性。随着时间的推移，从 hPSC 中重新生成器官型内皮细胞引起了争议。这篇透视文章重点介绍了 hPSC 来源的脑内皮细胞领域的进展、实验数据的缺乏以及自最初描述以来出现的问题。
Brain microvascular endothelial cells (BMECs) possess unique properties that are crucial for many functions of the blood-brain-barrier (BBB) including maintenance of brain homeostasis and regulation of interactions between the brain and immune system. The generation of a pure population of putative brain microvascular endothelial cells from human pluripotent stem cell sources (iBMECs) has been described to meet the need for reliable and reproducible brain endothelial cells in vitro. Human pluripotent stem cells (hPSCs), embryonic or induced, can be differentiated into large quantities of specialized cells in order to study development and model disease. These hPSC-derived iBMECs display endothelial-like properties, such as tube formation and low-density lipoprotein uptake, high transendothelial electrical resistance (TEER), and barrier-like efflux transporter activities. Over time, the de novo generation of an organotypic endothelial cell from hPSCs has aroused controversies. This perspective article highlights the developments made in the field of hPSC derived brain endothelial cells as well as where experimental data are lacking, and what concerns have emerged since their initial description.
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DOI：
10.1073/pnas.1019761108
发表时间：
2011-02-15
影响因子：
11.1
作者：
Anderson, Keith D.;Pan, Li;Gale, Nicholas W.
通讯作者：
Gale, Nicholas W.
影响因子：
7.2
作者：
Daneman, Richard;Prat, Alexandre
通讯作者：
Prat, Alexandre
影响因子：
5.9
作者：
Appelt-Menzel A;Cubukova A;Günther K;Edenhofer F;Piontek J;Krause G;Stüber T;Walles H;Neuhaus W;Metzger M
通讯作者：
Metzger M
影响因子：
64.8
作者：
Ben-Zvi, Ayal;Lacoste, Baptiste;Kur, Esther;Andreone, Benjamin J.;Mayshar, Yoav;Yan, Han;Gu, Chenghua
通讯作者：
Gu, Chenghua
影响因子：
64.8
作者：
Armulik, Annika;Genove, Guillem;Betsholtz, Christer
通讯作者：
Betsholtz, Christer