Expression of Human Metallothionein III and Its Metalloabsorption Capability in Escherichia coli
Expression of Human Metallothionein III and Its Metalloabsorption Capability in Escherichia coli
复制标题
人金属硫蛋白III在大肠杆菌中的表达及其金属吸收能力
DOI：
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发表时间：
2004
影响因子：
2.9
通讯作者：
Z. Hua
中科院分区：
文献类型：
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作者：
Wei;Fang;Hongqin Zhuang;Chun Lu;Feng Yang;Wen;Z. Hua
摘要利用大肠杆菌偏好密码子合成了人金属硫蛋白III(MT III)基因，并在大肠杆菌中以谷胱甘肽-S转移酶融合的形式表达。重组MT III经蛋白酶因子Xa消化后得到纯化，产量为2 mg/L培养物，证实了其与Cd~(2+)的结合能力。表达MT III的大肠杆菌BL21(DE3)对0.1~0.5 mM的Cd~(2+)具有耐受性，1 mM的Cd~(2+)对细菌生长有抑制作用。Mt III表达菌株在不同金属离子组合使用时表现出结合区分，其结合偏好顺序为：Cd~(2+)>；Cu2+>；它以相对恒定的比例吸附不同的金属离子，对混合重金属表现出累积吸附能力。
Abstract Human metallothionein III (MT III) gene was synthesized with Escherichia coli preference codon usage and expressed in E. coli in glutathione‐S‐transferase (GST) fusion form. The recombinant MT III was released by proteinase Factor Xa digestion and purified with the yield of 2 mg/L culture, and its specific Cd2+ binding capability was confirmed. E. coli strain BL21(DE3), expressing MT III, showed metal tolerance between 0.1 and 0.5 mM Cd2+ and bacterial growth was inhibited at 1 mM Cd2+. MT III expressing E. coli strain showed binding discrimination between different metal ions in combination use, with the preference order of Cd2+ > Cu2+ > Zn2+. It absorbed different metal ions with relatively constant ratio and showed a cumulative absorption capability for mixed heavy metals.
影响因子：
2.9
作者：
A. Sewell;L. T. Jensen;Jay C. Erickson;R. Palmiter;D. Winge
通讯作者：
A. Sewell;L. T. Jensen;Jay C. Erickson;R. Palmiter;D. Winge
DOI：
10.1073/pnas.89.14.6333
发表时间：
1992-07-15
影响因子：
11.1
作者：
PALMITER, RD;FINDLEY, SD;DURNAM, DM
通讯作者：
DURNAM, DM