Differentiating PC12 cells to evaluate neurite densities through live-cell imaging.
Differentiating PC12 cells to evaluate neurite densities through live-cell imaging.
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DOI：
10.1016/j.xpro.2022.101993
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发表时间：
2023-03-17
期刊：
影响因子：
--
通讯作者：
Merry, Diane E.
中科院分区：
文献类型：
--
作者：
Karliner, Jordyn;Merry, Diane E.
Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques. Differentiate PC12 cells into neuronal cells with equivalent neurite densities Use live-cell imaging to track PC12 cell differentiation over time Quantify neurite densities to establish time point for secondary analyses Validate differentiation by Western blot and immunostaining for neuronal markers Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques.
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影响因子：
5.4
作者：
Hu R;Cao Q;Sun Z;Chen J;Zheng Q;Xiao F
通讯作者：
Xiao F
影响因子：
3
作者：
Schwieger, Jana;Esser, Karl-Heinz;Scheper, Verena
通讯作者：
Scheper, Verena
影响因子：
3
作者：
Ohuchi, T;Maruoka, S;Arai, T
通讯作者：
Arai, T
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert
DOI：
10.1007/978-1-62703-438-8_18
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Cooper, Lori J;Merry, Diane E
通讯作者：
Merry, Diane E