A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium.
A novel method of neural differentiation of PC12 cells by using Opti-MEM as a basic induction medium.
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DOI：
10.3892/ijmm.2017.3195
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发表时间：
2018-01
影响因子：
5.4
通讯作者：
Xiao F
中科院分区：
文献类型：
--
作者：
Hu R;Cao Q;Sun Z;Chen J;Zheng Q;Xiao F
The PC12 cell line is a classical neuronal cell model due to its ability to acquire the sympathetic neurons features when deal with nerve growth factor (NGF). In the present study, the authors used a variety of different methods to induce PC12 cells, such as Opti-MEM medium containing different concentrations of fetal bovine serum (FBS) and horse serum compared with RPMI-1640 medium, and then observed the neurite length, differentiation, adhesion, cell proliferation and action potential, as well as the protein levels of axonal growth-associated protein 43 (GAP-43) and synaptic protein synapsin-1, among other differences. Compared with the conventional RPMI-1640 medium induction method, the new approach significantly improved the neurite length of induced cells (2.7 times longer), differentiation rate (30% increase), adhesion rate (21% increase) and expression of GAP-43 and synapsin-1 (three times), as well as reduced cell proliferation. The morphology of induced cells in Opti-MEM medium containing 0.5% FBS was more like that of neurons. Additionally, induced cells were also able to motivate the action potential after treatment for 6 days. Therefore, the research provided a novel, improved induction method of neural differentiation of PC12 cells using Opti-MEM medium containing 0.5% FBS, resulting in a better neuronal model cell line that can be widely used in neurobiology and neuropharmacology research.
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影响因子：
3.7
作者：
Medina Benavente JJ;Mogami H;Sakurai T;Sawada K
通讯作者：
Sawada K
DOI：
10.1155/2012/320308
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
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作者：
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通讯作者：
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影响因子：
4.8
作者：
Ramakers, GMJ;Gerendasy, DD;de Graan, PNE
通讯作者：
de Graan, PNE
影响因子：
16.2
作者：
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通讯作者：
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