X-ray diffraction from a single layer of purple membrane at the air/water interface.
X-ray diffraction from a single layer of purple membrane at the air/water interface.
复制标题
空气/水界面处单层紫色膜的 X 射线衍射。
DOI：
10.1006/jmbi.1999.2644
复制
发表时间：
1999
影响因子：
5.6
通讯作者：
M. Lösche
中科院分区：
文献类型：
--
作者：
S. Verclas;P. Howes;K. Kjaer;A. Wurlitzer;M. Weygand;G. Büldt;N. Dencher;M. Lösche
在Langmuir槽的空气/水界面处记录了单层紫色膜（约50 a厚）的x射线衍射图。掠入射x射线衍射被证明是在生理条件下获得膜蛋白结构信息的一种很有前途的方法。这种方法非常灵敏，光束中只有10（13）个蛋白质分子就可以测量样品的衍射。晶格常数为61的紫膜六方晶体的衍射。在{h，k}={4,3}阶上观测到3 A，对应的分辨率约为9 A。本文报道的工作是在空气/水界面上使用掠入射x射线衍射研究蛋白质晶体学新方法的第一步。
X-ray diffraction patterns have been recorded from a single layer of purple membrane ( approximately 50 A thickness) at the air/water interface in a Langmuir trough. Grazing-incidence X-ray diffraction is demonstrated to be a promising method for obtaining structural information on membrane proteins under physiological conditions. The method is so sensitive that diffraction can be measured from samples with only 10(13) protein molecules in the beam. Diffraction from hexagonal crystals of purple membrane with a lattice constant of 61. 3 A was observed up to the order {h,k}={4,3}, corresponding to a resolution of approximately 9 A. The work reported here is a first step towards a new way of protein crystallography using grazing-incidence X-ray diffraction at the air/water interface.
酶-效应子复合物的二维晶体：分辨率为 18-A 的核糖核苷酸还原酶。
影响因子：
2.9
作者：
Ribi,HO;Reichard,P;Kornberg,RD
通讯作者：
Kornberg,RD
影响因子：
5.6
作者：
HENDERSON, R;BALDWIN, JM;DOWNING, KH
通讯作者：
DOWNING, KH
影响因子：
3.4
作者：
DARST, SA;AHLERS, M;KORNBERG, RD
通讯作者：
KORNBERG, RD