A palmitoyltransferase Approximated gene Bm-app regulates wing development in Bombyx mori.
A palmitoyltransferase Approximated gene Bm-app regulates wing development in Bombyx mori.
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棕榈酰转移酶近似基因 Bm-app 调节家蚕翅膀发育
DOI：
10.1111/1744-7917.12629
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发表时间：
2020-02
期刊：
影响因子：
4
通讯作者：
Li MW
中科院分区：
文献类型：
--
作者：
Yu Y;Liu XJ;Ma X;Zhang ZJ;Wang TC;Sun F;Hou CX;Li MW
家蚕是一种重要的鳞翅目模式昆虫，已鉴定出多种自然突变体。然而，大多数这些突变的分子机制仍有待探索。在这里，我们报道了一个基因Bm‐app的鉴定，该基因负责家蚕微小翅膀（mw）突变，在蛹和成虫阶段表现出极小的翅膀。与野生型家蚕相比，u11突变株中Bm‐app的相对信使RNA表达量显著降低，表现为mw表型。在mw突变株的Bm‐app基因的假定启动子区域发现了一个10 bp的插入，双荧光素酶测定显示该插入降低了Bm‐app启动子的活性。此外，聚集的规律间隔的短回文重复序列/RNA引导的Cas9核酸酶介导的Bm‐app缺失诱导了类似的翅膀缺陷，这种缺陷出现在mw突变体中，这表明Bm‐app控制了家蚕的翅膀发育。Bm‐app编码一种棕榈酰转移酶，负责选定的细胞质蛋白的棕榈酰化，这表明在翅膀发育过程中，它是细胞有丝分裂和生长所必需的。我们还讨论了Bm‐app通过Hippo信号通路调控家蚕翅膀发育的可能性。
The silkworm Bombyx mori is an important lepidopteran model insect in which many kinds of natural mutants have been identified. However, molecular mechanisms of most of these mutants remain to be explored. Here we report the identification of a gene Bm‐app is responsible for the silkworm minute wing (mw) mutation which exhibits exceedingly small wings during pupal and adult stages. Compared with the wild type silkworm, relative messenger RNA expression of Bm‐app is significantly decreased in the u11 mutant strain which shows mw phenotype. A 10 bp insertion in the putative promoter region of the Bm‐app gene in mw mutant strain was identified and the dual luciferase assay revealed that this insertion decreased Bm‐app promoter activity. Furthermore, clustered regularly interspaced short palindromic repeats/RNA‐guided Cas9 nucleases‐mediated depletion of the Bm‐app induced similar wing defects which appeared in the mw mutant, demonstrating that Bm‐app controls wing development in B. mori. Bm‐app encodes a palmitoyltransferase and is responsible for the palmitoylation of selected cytoplasmic proteins, indicating that it is required for cell mitosis and growth during wing development. We also discuss the possibility that Bm‐app regulates wing development through the Hippo signaling pathway in B. mori.
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影响因子：
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