Protective effect of quercetin, EGCG, catechin and betaine against oxidative stress induced by ethanol in vitro.
Protective effect of quercetin, EGCG, catechin and betaine against oxidative stress induced by ethanol in vitro.
复制标题
槲皮素、EGCG、儿茶素和甜菜碱对体外乙醇诱导的氧化应激的保护作用。
DOI：
10.1016/j.yexmp.2011.02.006
复制
发表时间：
2011-06
影响因子：
3.6
通讯作者：
French, Samuel W.
中科院分区：
文献类型：
--
作者：
Oliva, Joan;Bardag-Gorce, Fawzia;Tillman, Brittany;French, Samuel W.
目前需要一种无毒的抗氧化剂来预防酒精中毒患者的酒精性肝病。我们测试了4种候选试剂：槲皮素，EGCG，儿茶素和甜菜碱，所有这些都天然存在于食物中。将过表达CYP 2 E1的HepG 2细胞置于花生四烯酸、铁和100 mM乙醇（含或不含抗氧化剂）中。除EGCG外，所有药物均能防止乙醇诱导的氧化应激和MDA/4 HNE形成。儿茶素可抑制乙醇对CYP 2 E1的诱导。所有的代理商倾向于下调乙醇诱导的谷胱甘肽过氧化物酶4（GPX 4）的表达增加。除儿茶素外，所有药物都有降低乙醇诱导的SOD 2表达的趋势。热休克蛋白70上调乙醇单独和甜菜碱倾向于防止这一点。所有4种药物在乙醇存在下下调Gadd 45 b的表达，这可以解释与实验性ALD中观察到的基因表达上调相关的DNA去甲基化机制。总之，乙醇诱导的氧化应激的体外模型提供了证据，证明所有4种测试药物都可以预防乙醇引起的肝细胞损伤的某些方面。
There is a need for a nontoxic antioxidant agent to be identified which will prevent alcoholic liver disease (ALD) in alcoholic patients. We tested 4 candidate agents: quercetin, EGCG, catechin and betaine, all of which occur naturally in food. HepG2 cells over expressing CYP2E1 were subjected to arachidonic acid, iron and 100 mM ethanol with or without the antioxidant agent. All the agents prevented oxidative stress and MDA/4HNE formation induced by ethanol, except for EGCG. Catechin prevented CYP2E1 induction by ethanol. All the agents tended to down regulate the ethanol-induced increased expression of glutathionine peroxidase 4 (GPX4). All the agents, except catechin, tended to reduce the expression of SOD2 induced by ethanol. Heat shock protein 70 was up regulated by ethanol alone and betaine tended to prevent this. All 4 agents down regulated the expression of Gadd45b in the presence of ethanol, which could explain the mechanism of DNA demethylation associated with the up regulation of the gene expression observed in experimental ALD. In conclusion, the in vitro model of oxidative stress induced by ethanol provided evidence that all 4 agents tested prevented some aspect of liver cell injury caused by ethanol.
登录
查看更多内容
影响因子：
13.5
作者：
Gonzalez, Oscar;Fontanes, Vanessa;Raychaudhuri, Santanu;Loo, Rachel;Loo, Joseph;Arumugaswami, Vaithilingaraja;Sun, Ren;Dasgupta, Asim;French, Samuel W.
通讯作者：
French, Samuel W.
影响因子：
3.7
作者：
Chen, X;Yin, OQP;Chow, MSS
通讯作者：
Chow, MSS
影响因子：
3.2
作者：
Liu, Shuang;Hou, Wei;Liu, Liegang
通讯作者：
Liu, Liegang
影响因子：
3.6
作者：
Bardag-Gorce, Fawzia;French, Barbara A.;French, Samuel W.
通讯作者：
French, Samuel W.
影响因子：
4.3
作者：
Kharbanda, Kusum K.;Todero, Sandra L.;Tuma, Dean J.
通讯作者：
Tuma, Dean J.