Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp

Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp
复制标题

23S rRNA 基因的系统发育和分子特征将弯曲杆菌属定位在变形菌门的 epsilon 细分中,并表明转录间隔区的存在在弯曲杆菌属中很常见

DOI:
--
复制
发表时间:
1994
影响因子:
3.2
通讯作者:
Robin Gutell
Robin Gutell
中科院分区:
生物学3区
文献类型:
--
作者:
T. Trust;S. Logan;Corinne E. Gustafson;P. Romaniuk;N. W. Kim;V. Chan;M. Ragan;P. Guerry;Robin Gutell

文献摘要

参考文献

被引文献

相似文献

测定了大肠弯曲杆菌VC 167的23S rRNA基因的核苷酸序列。C. coli23SrRNA序列,并构建二级结构模型。与大肠杆菌23S rRNA比较,C. colirRNA约1170位(E. coli编号),其形式为约147 bp长的额外序列块。对31株其它C. coli和C.空肠显示69%携带CA或CA的转录间隔区。147、你37 bp。所有测序的弯曲杆菌转录间隔区的比较表明,弯曲杆菌插入相关的序列和百分比G+C含量。所有在其23S rRNA基因中携带转录间隔区的弯曲杆菌菌株产生片段化的23S rRNA。其他产生未片段化23S rRNA的菌株似乎在其23S rRNA基因的该位置不携带转录间隔区。在1850年(E。大肠杆菌编号),弯曲杆菌23S rRNA显示出与变形菌纲的β和γ亚门最相似的碱基配对特征,但在270区域,弯曲杆菌23S rRNA显示出将其与α、β和γ亚门区分开的螺旋特征。系统发育分析表明,C. coliVC 167 23SrRNA和C. jejuni TGH9011(ATCC 43431)23S rRNA与53个其他完全测序(eu)的细菌23S rRNA的比较显示,两种弯曲杆菌与α、β和γ变形菌23S rRNA形成姐妹群,这一定位与弯曲杆菌属属于变形菌纲的亚门的观点一致。
The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. coli numbering) in the form of an extra sequence block approximately 147 bp long. PCR analysis of 31 other strains of C. coli and C. jejuni showed that 69% carried a transcribed spacer of either ca. 147 or ca. 37 bp. Comparison of all sequenced Campylobacter transcribed spacers showed that the Campylobacter inserts were related in sequence and percent G+C content. All Campylobacter strains carrying transcribed spacers in their 23S rRNA genes produced fragmented 23S rRNAs. Other strains which produced unfragmented 23S rRNAs did not appear to carry transcribed spacers at this position in their 23S rRNA genes. At the 1850 region (E. coli numbering), Campylobacter 23S rRNA displayed a base pairing signature most like that of the beta and gamma subdivisions of the class Proteobacteria, but in the 270 region, Campylobacter 23S rRNA displayed a helix signature which distinguished it from the alpha, beta, and gamma subdivisions. Phylogenetic analysis comparing C. coli VC167 23S rRNA and a C. jejuni TGH9011 (ATCC 43431) 23S rRNA with 53 other completely sequenced (eu)bacterial 23S rRNAs showed that the two campylobacters form a sister group to the alpha, beta, and gamma proteobacterial 23S rRNAs, a positioning consistent with the idea that the genus Campylobacter belongs to the epsilon subdivision of the class Proteobacteria.
DOI: 10.1006/jmbi.1993.1402
发表时间: 1993
影响因子: 5.6
作者:
Turmel,M;Gutell,RR;Mercier,JP;Otis,C;Lemieux,C
通讯作者: Lemieux,C
大肠杆菌 K12 基因组的物理图谱。
DOI: 10.1126/science.3296194
发表时间: 1987
期刊: Science (New York, N.Y.)
影响因子: --
作者:
Smith,CL;Econome,JG;Schutt,A;Klco,S;Cantor,CR
通讯作者: Cantor,CR
几种系统发育推断方法在不同进化速率下的一致性。
DOI: 10.1093/oxfordjournals.molbev.a040740
发表时间: 1992
影响因子: 10.7
作者:
DeBry,RW
通讯作者: DeBry,RW
弯曲杆菌属的系统发育多样性和位置。
DOI: 10.1016/s0723-2020(87)80027-9
发表时间: 1987
影响因子: 3.4
作者:
Lau,PP;DeBrunner-Vossbrinck,B;Dunn,B;Miotto,K;MacDonnell,MT;Rollins,DM;Pillidge,CJ;Hespell,RB;Colwell,RR;Sogin,ML;Fox,GE
通讯作者: Fox,GE
DOI: 10.1093/nar/20.21.5785
发表时间: 1992-11-11
影响因子: 14.9
作者:
GUTELL, RR;POWER, A;STORMO, GD
通讯作者: STORMO, GD