Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp
Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp
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23S rRNA 基因的系统发育和分子特征将弯曲杆菌属定位在变形菌门的 epsilon 细分中,并表明转录间隔区的存在在弯曲杆菌属中很常见
DOI:
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发表时间:
1994
影响因子:
3.2
通讯作者:
Robin Gutell
中科院分区:
文献类型:
--
作者:
T. Trust;S. Logan;Corinne E. Gustafson;P. Romaniuk;N. W. Kim;V. Chan;M. Ragan;P. Guerry;Robin Gutell
The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. coli numbering) in the form of an extra sequence block approximately 147 bp long. PCR analysis of 31 other strains of C. coli and C. jejuni showed that 69% carried a transcribed spacer of either ca. 147 or ca. 37 bp. Comparison of all sequenced Campylobacter transcribed spacers showed that the Campylobacter inserts were related in sequence and percent G+C content. All Campylobacter strains carrying transcribed spacers in their 23S rRNA genes produced fragmented 23S rRNAs. Other strains which produced unfragmented 23S rRNAs did not appear to carry transcribed spacers at this position in their 23S rRNA genes. At the 1850 region (E. coli numbering), Campylobacter 23S rRNA displayed a base pairing signature most like that of the beta and gamma subdivisions of the class Proteobacteria, but in the 270 region, Campylobacter 23S rRNA displayed a helix signature which distinguished it from the alpha, beta, and gamma subdivisions. Phylogenetic analysis comparing C. coli VC167 23S rRNA and a C. jejuni TGH9011 (ATCC 43431) 23S rRNA with 53 other completely sequenced (eu)bacterial 23S rRNAs showed that the two campylobacters form a sister group to the alpha, beta, and gamma proteobacterial 23S rRNAs, a positioning consistent with the idea that the genus Campylobacter belongs to the epsilon subdivision of the class Proteobacteria.
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影响因子:
5.6
作者:
Turmel,M;Gutell,RR;Mercier,JP;Otis,C;Lemieux,C
通讯作者:
Lemieux,C
DOI:
10.1126/science.3296194
发表时间:
1987
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
Smith,CL;Econome,JG;Schutt,A;Klco,S;Cantor,CR
通讯作者:
Cantor,CR
影响因子:
10.7
作者:
DeBry,RW
通讯作者:
DeBry,RW
影响因子:
3.4
作者:
Lau,PP;DeBrunner-Vossbrinck,B;Dunn,B;Miotto,K;MacDonnell,MT;Rollins,DM;Pillidge,CJ;Hespell,RB;Colwell,RR;Sogin,ML;Fox,GE
通讯作者:
Fox,GE
影响因子:
14.9
作者:
GUTELL, RR;POWER, A;STORMO, GD
通讯作者:
STORMO, GD