The Drosophila PGC-1α homolog spargel modulates the physiological effects of endurance exercise.
The Drosophila PGC-1α homolog spargel modulates the physiological effects of endurance exercise.
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DOI：
10.1371/journal.pone.0031633
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wessells RJ
中科院分区：
文献类型：
--
作者：
Tinkerhess MJ;Healy L;Morgan M;Sujkowski A;Matthys E;Zheng L;Wessells RJ
耐力运动是一种廉价的干预措施，被认为可以提供实质性的保护，防止几种与年龄有关的病理，以及诱导耐力和新陈代谢的急性变化。最近，它已被确定为耐力运动诱导无脊椎动物果蝇模型中的生理能力的保守的改变。如果这些运动引起的生理变化的遗传因素是广泛保守的，那么无脊椎动物遗传模型系统将成为测试耐力训练的遗传和药理模拟物的有价值的工具。在这里，我们评估是否果蝇同源的脊椎动物运动反应基因PGC-1α spargel（srl）是必要的或足以诱导运动依赖性表型。我们发现，减少srl表达水平严重损害负趋地性能力，并减少运动诱导的改善负趋地性和时间耗尽。相反，肌肉/心脏特异性srl过表达改善了未运动果蝇的负趋地性和心脏性能。此外，我们发现srl的过度表达模拟了一些但不是全部运动诱导的表型，这表明其他因素也与srl平行作用，以调节运动诱导的肌肉和心脏的生理变化。
Endurance exercise is an inexpensive intervention that is thought to provide substantial protection against several age-related pathologies, as well as inducing acute changes to endurance capacity and metabolism. Recently, it has been established that endurance exercise induces conserved alterations in physiological capacity in the invertebrate Drosophila model. If the genetic factors underlying these exercise-induced physiological alterations are widely conserved, then invertebrate genetic model systems will become a valuable tool for testing of genetic and pharmacological mimetics for endurance training. Here, we assess whether the Drosophila homolog of the vertebrate exercise response gene PGC-1α spargel (srl) is necessary or sufficient to induce exercise-dependent phenotypes. We find that reduction of srl expression levels acutely compromises negative geotaxis ability and reduces exercise-induced improvement in both negative geotaxis and time to exhaustion. Conversely, muscle/heart specific srl overexpression improves negative geotaxis and cardiac performance in unexercised flies. In addition, we find that srl overexpression mimics some, but not all, exercise-induced phenotypes, suggesting that other factors also act in parallel to srl to regulate exercise-induced physiological changes in muscle and heart.
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DOI：
10.1249/01.mss.0000183179.86594.4f
发表时间：
2006-02-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
Davidson, SR;Burnett, M;Hoffman-Goetz, L
通讯作者：
Hoffman-Goetz, L
影响因子：
8.3
作者：
Cornelissen, VA;Fagard, RH
通讯作者：
Fagard, RH
影响因子：
64.5
作者：
Puigserver, P;Wu, ZD;Spiegelman, BM
通讯作者：
Spiegelman, BM
影响因子：
64.5
作者：
Lin, JD;Wu, PH;Spiegelman, BM
通讯作者：
Spiegelman, BM
影响因子：
4.8
作者：
Wright, David C.;Han, Dong-Ho;Holloszy, John O.
通讯作者：
Holloszy, John O.