Real-time detection of the morphological change in cellulose by a nanomechanical sensor.
Real-time detection of the morphological change in cellulose by a nanomechanical sensor.
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DOI：
10.1002/bit.22754
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发表时间：
2010-09-01
影响因子：
3.8
通讯作者：
Xi, Jun
中科院分区：
文献类型：
--
作者：
Zhao, Liming;Bulhassan, Ahmed;Yang, Guoliang;Ji, Hai-Feng;Xi, Jun
到目前为止，实验局限性阻止了研究人员对纤维素的酶促水解的初始步骤实现分子水平的理解，在纤维素中，纤维素酶破坏了纤维素表面的晶体结构，并暴露了纤维素链的晶体结构，并通过纤维素酶进行水解纤维素链的水解。 。因为这些非溶解酶促步骤之一可能是通过纤维素酶通过晶体纤维素进行整个酶水解的速率限制步骤，因此能够分析和理解这些步骤在发现新的铅方面对提高纤维素酶效率的新铅具有重要作用。在这种交流中，我们报告了微型管理器技术的创新应用，以实时评估氯化钠治疗诱导的纤维素的形态变化。这种敏感的纳米力学方法来定义纤维素表面结构的变化，有可能实时评估纤维素纤维素的非溶解活性对纤维素的影响，从而提供对酶水解的初始步骤的全面理解。纤维素。
Up to now, experimental limitations have prevented researchers from achieving the molecular-level understanding for the initial steps of the enzymatic hydrolysis of cellulose, where cellulase breaks down the crystal structure on the surface region of cellulose and exposes cellulose chains for the subsequent hydrolysis by cellulase. Because one of these non-hydrolytic enzymatic steps could be the rate-limiting step for the entire enzymatic hydrolysis of crystalline cellulose by cellulase, being able to analyze and understand these steps is instrumental in uncovering novel leads for improving the efficiency of cellulase. In this communication, we report an innovative application of the microcantilever technique for a real-time assessment of the morphological change of cellulose induced by a treatment of sodium chloride. This sensitive nanomechanical approach to define changes in surface structure of cellulose has the potential to permit a real time assessment of the effect of the non-hydrolytic activities of cellulase on cellulose and thereby to provide a comprehensive understanding of the initial steps of the enzymatic hydrolysis of cellulose.
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